eng Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2022-04-18 10 4 305 312 10.12691/jfnr-10-4-7 JFNR20221047 article Yung-Kai Lin 1 2 3 Chia-Hua Liang 4 Yung-Hsiang Lin 5 Shu-Ting Chan 5 Chi-Fu Chiang Jimmy.Chiang@tci-bio.com 5 Institute of Food Safety and Risk Management, National Taiwan Ocean University, Keelung, Taiwan Department of Cosmetic Science and Institute of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan, Taiwan Research & Design Center, TCI CO., Ltd., Taipei, Taiwan Background: As the population ages, oral hydrolyzed collagen supplements are gaining traction. However, few clinical studies on the skin health of hydrolyzed porcine collagen combined with fish collagen. Objective: This study investigates whether TripleUp collagen beverage has skincare and anti-inflammatory effects. Methods: Using collagen beverage to treat epidermal keratinocytes and examine ROS, HA, inflammatory factor, and moisturizing-related gene expressions (Tgm1, Krt14, FLG-F, GBA, HAS3). Fifty subjects were recruited into a placebo and collagen beverage group for four weeks and then examined skin condition at 0, 2, 4 weeks. Results: TripleUp collagen beverage decreased IL-8 and ROS expression and increased moisturizing-related gene expressions. In addition, collagen beverages increase skin collagen, elasticity, moisture, and decreased spots. Conclusions: Thus, TripleUp collagen beverage included hydrolyzed porcine, and fish collagen and vitamin C can improve skin condition. http://pubs.sciepub.com/jfnr/10/4/7/jfnr-10-4-7.pdf collagen fish skin porcine vitamin C