@article{jfnr20221023,
author={{Elsharawy, Nagwa Thabet and Al-Zahrani, Hind A. A. and El-Waseif, Amr A.},
title={Phenotypic and Genotypic Characterization of Antimicrobial Resistance in <i>Escherichia Coli</i> Isolates from Chicken Meat},
journal={Journal of Food and Nutrition Research},
volume={10},
number={2},
pages={98--104},
year={2022},
url={http://pubs.sciepub.com/jfnr/10/2/3},
issn={2333-1240},
abstract={Improper use of the antimicrobials as <i>E. coli </i>giving the microorganism multi-resistance against many antimicrobials by gene mutation on integrons, transposons and plasmids. Therefore, our aim in this study is to 1) examine antibiotics resistance phenotype and genotype in <i>Escherichia</i><i> </i><i>coli</i>, 2) identifying the structure of bacterial resistance genes on whole-genome sequencing against multi-drug resistant of <i>Escherichia coli </i>in marketed poultry meat. Samples collected, prepared and Bacteriological examination, Antimicrobial sensitivity test performed, Serological identification of <i>Escherichia coli </i>isolates. Results declared that; the prevalence of <i>E. coli</i><i> </i>from tested chicken meat samples of 100 chicken meat samples surveyed against <i>E. coli </i>the result declared that about 40%<i>.</i><i> </i>Antimicrobial susceptibility was; antibiotics of choice against <i>E.</i><i> </i><i>coli</i><i> </i>Sulfonamides, Cephalosporins, Tetracyclines, Quinolones. Serologically, STEC (O157:H7) 30%, ETEC (O142) 10%, EHEC (O26:H11). The subunit B of shiga-like toxin (SLT) gene appeared as a homogenous band. Heat-labile toxin (LT) gene was screened in both genomic DNA and plasmid preps in tested strains. Control STEC as it represents a danger to the poultry consumers. We recommended to increase the hygienic measures during slaughtering, processing and/or handling of chicken carcasses and avoidance unnecessary usage of any antimicrobials to avoid appearance of new antimicrobials resistant.},
doi={10.12691/jfnr-10-2-3}
publisher={Science and Education Publishing}
}