@article{jfnr2013132,
author={{Wang, Cong and Li, Shaoting and Chen, Long and Peng, Xichun and Wu, Xiyang},
title={The Response of Colonic <i>Clostridium</i> Cluster IV to Essential Oil and Aqueous Extract of <i>Cinnamon Cassia</i> in Rats},
journal={Journal of Food and Nutrition Research},
volume={1},
number={3},
pages={24--29},
year={2013},
url={http://pubs.sciepub.com/jfnr/1/3/2},
abstract={The multiple pharmacological effects of <i>Cinnamomum cassia</i> (<i>C. cassia</i>) have been reported. The aim of this paper is to test the<i> in vivo</i> response of <i>C. cassia</i> derived materials on colonic <i>Clostridium </i>cluster IV that is supposed to relate the energy metabolism in body. The essential oil and aqueous extract of <i>C. cassia</i> were orally administered to 2 tested groups of Sprague-Dawley (SD) rats for 30 days. After 30-days cultivation, the colonic contents of the rats were sampled to investigate the <i>Clostridium</i> cluster IV community structure through terminal restriction fragment length polymorphism (T-RFLP) and 16S rRNA gene clone library analysis. The results showed that the total peak areas of colonic <i>Clostridium</i> cluster IV, Shannon diversity index and evenness all decreased significantly in 2 tested groups compared with the control group (P &lt; 0.01). Jaccard similarity index was under 50% in both tested groups. Putative species corresponding to diet-associated terminal restriction fragments (TRFs), 89, 172 and 177 bp, were identified through gene sequence as <i>Acetanaerobacterium elongatum</i>, <i>Hydrogenoanaerobacterium saccharovorans</i>, <i>Anaerotruncus colihominis</i>,<i> Ethanoligenens harbinense</i>, <i>Clostridium cellulosi</i>, <i>Clostridium orbiscindens</i>, <i>Ruminococcus</i> <i>bromii </i>and <i>Papillibacter cinnamivorans</i>. In conclusions, both essential oil and aqueous extract of<i> C. cassia </i>can alter the bacterial community structure and decline the diversity of colonic <i>Clostridium</i> cluster IV in SD rats. Those species of <i>Clostridium</i> cluster IV impacted during the experiment may play a role in the energy metabolism of host.},
doi={10.12691/jfnr-1-3-2}
publisher={Science and Education Publishing}
}