@article{jcrt2018633,
author={{Khalil, Ahmad M. and Alghadi, Ahmad Y. and Shahen, Rahaf M. T. and Elasad, Jehad W. and Jawasreh, Khaleel I.},
title={Inhibition of the Inositol Requiring Protein 1¦Á- X-Box Binding Protein-1 Pathway as a Promising Therapeutic Target for Human Prostate Cancer},
journal={Journal of Cancer Research and Treatment},
volume={6},
number={3},
pages={74--79},
year={2018},
url={http://pubs.sciepub.com/jcrt/6/3/3},
issn={2374-2003},
abstract={Prostate cancer (PCa) has been associated with endoplasmic reticulum stress (ERS) which activates the inositol requiring protein 1¦Á- X-box binding protein-1 (IRE¦Á-XBP-1) pathway. The aim of the study was to investigate the role of this pathway in three human PCa cell lines (LNCaP, PC-3, and DU-145) by evaluating the expression of XBP-1 and glucose-regulated protein 78 (GRP78) genes. The effect of two ERS inducers (Thapsigargin, Tg and tunicamycin, Tm) alone and in combination with an inhibitor of the IRE1¦Á RNase inhibitor (STF-083010) on expression profiling was followed using Quantitative-PCR. In vitro treatment of PCa cells with ERS inducers upregulated expression of XBP-1 gene. STF-083010 inhibited IRE1¦Á-induced splicing of the gene and increased cytotoxicity. Inhibition of IRE1¦Á RNase activity significantly decreased expression of chaperon protein GRP78. The results confirm and extend the concept that selective targeting of IRE1¦Á-XBP-1 pathway might be a novel therapeutic approach that curbs PCa cell progression.},
doi={10.12691/jcrt-6-3-3}
publisher={Science and Education Publishing}
}