Science and Education Publishing Journal of Cancer Research and Treatment 2 1 2014 01 03 Study of p53, Pcna, Ki67 and Micronuclei Related to Genotoxic Damage for Risk Categorization in Pre-Malignant Oral Lesions 10 15 EN Asoke Roy Department of Pathology and Cervical Screening, Chittaranjan National Cancer Institute, Kolkata, India Satyendra Prakash Bhatnagar Malay Chatterjee Dipanwita Ghosh Goutam Mandal Shyamsundar Mondal Shyamal Goswami JCRT2014213 10.12691/jcrt-2-1-3 2013 11 29 2013 12 19 2014 01 03 In this prospective study, search for high risk cases of premalignant oral lesions were attempted through evaluation of oncoprotein expression, cell proliferation and micronuclei. Materials and methods: A total 50 cases of oral leukoplakia were diagnosed and adequate controls along with detailed history were included in this study. Study of tumour markers like p53, PCNA and Ki-67 were done immunohistochemically on tissue sections. Study of micronuclei was performed by feulgen staining method. Results: The mean age of the 50 cases were 44.48 ± 9.76 and median age was 50 years. There were 45 male cases (90%) and 5 female cases (10%). There were 26 non dysplastic (52%) and 24 dysplastic cases (48%). The smokers consist of 25 cases (50%) and other addictions (betel quid chewers, ghutka, khaini, alcohol etc.) had 24 cases (48%) and 1 case was non addict (2%). Out of 25 cases of smokers, 20 (80%) were positive in case of p53, 19 (76%) were positive in case of PCNA and 17 (68%) were positive in case of Ki67. Out of 24 cases of other addictions, 16 (66.67%) cases were positive in case of p53, 13 (54.17%) positive cases in case of PCNA and16 cases (66.67%) were positive in case of Ki67.The smokers had 0.51 ± 0.21 micronuclei frequency whereas other addiction groups had 0.24 ± 0.10. Conclusion: The histopathological risk assessment of these cases is inconclusive and thus p53, PCNA, Ki67 and micronuclei evaluation are needed for risk categorization.