@article{jcrt2014213,
author={{Roy, Asoke and Bhatnagar, Satyendra Prakash and Chatterjee, Malay and Ghosh, Dipanwita and Mandal, Goutam and Mondal, Shyamsundar and Goswami, Shyamal},
title={Study of p53, Pcna, Ki67 and Micronuclei Related to Genotoxic Damage for Risk Categorization in Pre-Malignant Oral Lesions},
journal={Journal of Cancer Research and Treatment},
volume={2},
number={1},
pages={10--15},
year={2014},
url={http://pubs.sciepub.com/jcrt/2/1/3},
abstract={In this prospective study, search for high risk cases of premalignant oral lesions were attempted through evaluation of oncoprotein expression, cell proliferation and micronuclei. <i>Materials and methods:</i> A total 50 cases of oral leukoplakia were diagnosed and adequate controls along with detailed history were included in this study. Study of tumour markers like p53, PCNA and Ki-67 were done immunohistochemically on tissue sections. Study of micronuclei was performed by feulgen staining method. <i>Results:</i> The mean age of the 50 cases were 44.48 ¡À 9.76 and median age was 50 years. There were 45 male cases (90%) and 5 female cases (10%). There were 26 non dysplastic (52%) and 24 dysplastic cases (48%). The smokers consist of 25 cases (50%) and other addictions (betel quid chewers, ghutka, khaini, alcohol etc.) had 24 cases (48%) and 1 case was non addict (2%). Out of 25 cases of smokers, 20 (80%) were positive in case of p53, 19 (76%) were positive in case of PCNA and 17 (68%) were positive in case of Ki67. Out of 24 cases of other addictions, 16 (66.67%) cases were positive in case of p53, 13 (54.17%) positive cases in case of PCNA and16 cases (66.67%) were positive in case of Ki67.The smokers had 0.51 ¡À 0.21 micronuclei frequency whereas other addiction groups had 0.24 ¡À 0.10. <i>Conclusion:</i><b> </b>The histopathological risk assessment of these cases is inconclusive and thus p53, PCNA, Ki67 and micronuclei evaluation are needed for risk categorization.},
doi={10.12691/jcrt-2-1-3}
publisher={Science and Education Publishing}
}