@article{jaem2020814,
author={{Adama, OUATTARA and Klotioloma, COULIBALY and Ibrahim, KONATE and Isma?l, KEBE Boubacar and Maxwell, BEUGRE G.A. and Sanogo, TIDOU Abiba and FILALI-MALTOUF, Abdelkarim},
title={Screening and Selection <i>in vitro</i> and <i>in vivo</i> of Cocoa Tree (<i>Theobroma Cacao</i> Linn) Endophytic Bacteria Having Antagonistic Effects against <i>Phytophthora </i>Spp. Fungal Agents Responsible of Black Pod Disease in C?te d¡¯Ivoire},
journal={Journal of Applied & Environmental Microbiology},
volume={8},
number={1},
pages={25--31},
year={2020},
url={http://pubs.sciepub.com/jaem/8/1/4},
issn={2373-6712},
abstract={We are investigating in this study the screening and selection of cocoa tree (<i>Theobroma cacao</i> L.) endophytic bacteria having antagonistic effects against the fungi<i> Phytophthora</i> spp. and to assess their inhibitory potential as biological control agents against black pod disease in C?te d'Ivoire. A total of One hundred and sixteen bacteria were isolated from symptomless root, leaf and stem tissues of two cocoa clones NA32 (susceptible) and P7 (resistant) young seedlings. These isolates restricted the radial growth of the mycelium of <i>P. palmivora</i> (strain BL7.11.2) and <i>P. megakarya</i> (strain 13P30.1) with percentages inhibition of 28.58 to 70.54% and 25.3 to 64.29% respectively. Four isolates, 48P, 60P, 23P and 18N were more effective in <i>in vitro</i> test and selected for <i>in vivo</i> confrontation test on leaf discs. Treatments based on two isolates S1 (48P) and S2 (18N) applied at the concentration 10<SUP>9</SUP> CFU/mL significantly (p &lt; 0.05) reduced the black pod necrosis induced by the two <i>Phytophthora</i> strains on the leaf discs of three cocoa clones (NA32, PA150 and SCA6). In addition, simultaneous (C3PS1 and C3PS2) or separate (C3AVS1 and C3AVS2) inoculations of these two bacteria and <i>Phytophthora</i>, induced resistance to the clone NA32 and increased the intrinsic resistance of the clones PA150 and SCA6. This study showed that endophytic bacteria antagonistic to <i>Phytophthora</i> spp. can be an alternative solution in the biocontrol of black pod disease. <i>In situ</i> pod tests should be performed to confirm these preliminary results and propose effective bacterial inoculum.},
doi={10.12691/jaem-8-1-4}
publisher={Science and Education Publishing}
}