@article{jaem2017524,
author={{Alshubaith, I. and Gilmour, D. J.},
title={Isolation and Characterization of Moderately Halophilic and Halotolerant Bacteria from the Freshwater Al-Asfar Lake, Al Ahsa, Saudi Arabia},
journal={Journal of Applied & Environmental Microbiology},
volume={5},
number={2},
pages={79--85},
year={2017},
url={http://pubs.sciepub.com/jaem/5/2/4},
issn={2373-6712},
abstract={A range of water samples from the Al-Asfar lake, Al Ahsa, Saudi Arabia were enriched with LB medium (plates and liquid medium) containing a range of salt concentrations up to 2 M NaCl. Three strains of halotolerant or moderately halophilic bacteria were isolated and identified by 16S rDNA sequencing as belonging to the genera <i>Staphylococcus, Halobacillus </i>and <i>Halomonas.</i> The first two organisms (<i>S. warneri </i>and <i>Halobacillus </i>sp.) were further charcaterized to understand their ability to grow at high salinities up to 3 M NaCl. <i>Halobacillus </i>sp. was shown to be moderately halophilic (optimum growth between 0.17 and 1 M NaCl), whereas <i>S. warneri</i> was shown to be halotolerant, optimum growth at 0.17 M NaCl. Nuclear magnetic resonance (NMR) was used to determine the compatible solutes accumulated by the two strains. Betaine was accumulated by both organisms and <i>Halobacillus</i> sp. also utilized glutamate at low salt concentrations. This work demonstrates the presence of moderately halophilic and halotolerant bacteria in a freshwater lake.},
doi={10.12691/jaem-5-2-4}
publisher={Science and Education Publishing}
}