Science and Education Publishing Journal of Applied & Environmental Microbiology 2373-6712 3 4 2015 10 19 16S rDNA Identification of Arsenite-Oxidizing Bacillus sp. Isolated from Arsenic Contaminated Surface Water Uttar Pradesh, India 101 105 EN Bilal Ahmad Tantry Department of Microbiology, college of medicine, Aljouf University, Sakaka,Saudi Arabia Divya Shrivastava Ibrahim Taher Mudasar Nabi Tantry JAEM2015342 10.12691/jaem-3-4-2 2015 6 24 2015 9 10 2015 10 17 Three novel Arsenite oxidizing bacteria have been isolated from arsenic contaminated rivers from India. Samples were collected from the three districts of Uttar Pradesh (UP) viz., Ghaziabad, Moradabad and Gorakhpur Uttar Pradesh, India. All the three stains were observed as gram positive, aerobic, spore forming and rod shaped. The phylogentic analysis using 16S rDNA identification sequence showed that three isolates were belonged to the family of Bacillaceae and were closely related to Rummeliibacillus sp. (98%), Brevibacillus agri strain (88%) and Bacillus thuringiensis (99%). All the three strains are clearly distinguished from the related Bacillus species by its biochemical characteristics as well as the phylogentic relationship, therefore the three strains represent the novel sp. of the genus bacillus for which we proposed the names viz., Rummeliibacillus sp. AOBG-1, Brevibacillus sp. AOBMI-1 and Bacillus sp. AOB-GI-1 respectively. The GenBank accession numbers for the 16S rRNA gene sequence of the strains are KP295449, KP314033 and KP 308382.