@article{jaem2015324,
author={{K.F., Giddey and M., Kidd and T.J., Britz and G.O., Sigge and C., Lamprecht},
title={Impact of Hydrogen Peroxide Treatment on Environmental <i>Escherichia coli</i> Strains},
journal={Journal of Applied & Environmental Microbiology},
volume={3},
number={2},
pages={49--57},
year={2015},
url={http://pubs.sciepub.com/jaem/3/2/4},
issn={2373-6712},
abstract={The impact of three hydrogen peroxide (H<SUB>2</SUB>O<SUB>2</SUB>) concentrations (250, 300 and 350 mg.L<SUP>-1</SUP>) at 30, 60, 90 and 120 min time intervals was determined on environmental and ATCC reference <i>E. coli</i> strains (n=11). Variation between strains was evident and treatment resulted in significantly different log reductions after the 120 min contact time. The environmental strains were generally more resistant than the reference strains. A H<SUB>2</SUB>O<SUB>2</SUB> resistant environmental strain (M53) and a potential pathogenic strain (W1371) were used to determine bactericidal effect at higher (and a lower) H<SUB>2</SUB>O<SUB>2 </SUB>concentrations of 50, 350, 700 and 1 000 mg.L<SUP>-1</SUP> on the microbial inactivation. Bacterial inactivation increased as concentration increased, with 50 mg.L<SUP>-1</SUP> resulting in low microbial inactivation and 1 000 mg.L<SUP>-1</SUP> resulting in an effective (>4 log) reduction. A significant difference in microbial reduction was not observed at H<SUB>2</SUB>O<SUB>2</SUB> concentrations between 350 and 700 mg.L<SUP>-1</SUP>. The potential influence of the COD (chemical oxygen demand) of river water on the H<SUB>2</SUB>O<SUB>2 </SUB>treatment was also determined. It was observed that the water COD, at the levels investigated, might influence H<SUB>2</SUB>O<SUB>2 </SUB>efficacy<SUB> </SUB>treatment over shorter treatment times (30 min), but not over longer periods (90-120 min). Different levels of catalase activity were also measured for the test strains. A trend was observed between H<SUB>2</SUB>O<SUB>2</SUB> resistance and an increased amount of HPII catalase activity. However, it was also observed that <i>E. coli</i> can also employ other protection mechanisms, as two of the most resistant environmental <i>E. coli</i> strains only indicated average catalase activity.},
doi={10.12691/jaem-3-2-4}
publisher={Science and Education Publishing}
}