Science and Education Publishing Journal of Applied & Environmental Microbiology 2 5 2014 08 01 Purification and Analysis of Norcadia spp .Azoreductase 237 243 EN M. Shah Industrial Waste Water Research Laboratory Division of Applied & Environmental Microbiology Enviro Technology Limited Gujarat, India JAEM2014256 10.12691/jaem-2-5-6 2014 07 18 2014 07 25 2014 08 01 Nocardia spp. was isolated as Acid Red degrader. The reductive cleavage of azo bond was catalyzed by azo reductase, the key enzyme for the azo dye degradation. Azo reductase from the Nocardia spp. isolate was purified and characterized. Azoreductase was found to have the following features. The catalytic reduction of acid red 37 by purified azoreductase in the presence of NADH as electron donor was studied and the products of degradation were determined as 1-{3-amino-5-[(aminoxy)sulfonyl]phenyl}ethanol and 7,8-diamino-3[(aminoxy)sulfonyl]naphthalene-1-ol.