@article{jaem20142412,
author={Shah, M.},
title={Exploring the Efficacy of Bioaugmentation Strategy in Microbial Degradation of Chloroaniline},
journal={Journal of Applied & Environmental Microbiology},
volume={2},
number={4},
pages={185--193},
year={2014},
url={http://pubs.sciepub.com/jaem/2/4/12},
abstract={<i>Pseudomonas spp</i>. was isolated from activated-sludge and found to be able to mineralize 3-chloroaniline (3-CA). This strain was tested for its ability to clean wastewater containing 3-CA upon inoculation in activated-sludge. To monitor its survival, the strain was chromosomally marked with the <i>gfp </i>gene and designated A<i>gfp</i>. After inoculation into a lab-scale semi continuous activated-sludge (SCAS) system, the inoculated strain maintained itself in the sludge at least 45 days and was present in the sludge flocs. After an initial adaptation period of 6 days, complete degradation of 3-CA was obtained during two weeks, while no degradation at all occurred in the non-inoculated control reactors. Upon further operation of the SCAS system, only 50 % 3-CA removal was observed<i>. </i>Denaturing Gradient Gel Electrophoresis (DGGE) of 16S rRNA genes revealed a dynamic change in the microbial community structure of the activated-sludge. The DGGE patterns of the non inoculated and the inoculated reactors evolved after 7 days to different clusters, which suggests an effect of strain inoculation on the microbial community structure. The results indicate that bioaugmentation, even with a strain originating from that ecosystem and able to effectively grow on a selective substrate, is not permanent and will probably require regular resupplementation.},
doi={10.12691/jaem-2-4-12}
publisher={Science and Education Publishing}
}