@article{jaem2014217,
author={Hasan, Ihsan Flayyih},
title={Biodegradation of Kerosene by <i>Aspergillus </i><i>n</i><i>iger</i> and <i>Rhizopus </i><i>s</i><i>tolinifer</i>},
journal={Journal of Applied & Environmental Microbiology},
volume={2},
number={1},
pages={31--36},
year={2014},
url={http://pubs.sciepub.com/jaem/2/1/7},
abstract={This study investigated the abiliy of two fungi to utilize Kerosene. The fungal isolates obtained in this study were <i>Aspergillus</i><i> </i><i>niger </i>and<i> Rhizopus stolinifer</i><i>. </i>In the present study, a significant differences in the percent of Kerosene degrading fungi were evident among the time of biodegradation. The growth profiles were determined by monitoring growth ability in (potato dextrose agar PDA) medium containing 0.0, 5%, 10%, 15%, 20% v/v Kerosene, dry weights and pH of utilizing Kerosene as carbon and energy source were determined. There was no significant in dry weights of fungi at the 7 days of incubation. <i>A</i><i>.</i><i>niger</i><i> </i>had the highest dry weight value of 0.530 gm in 10% concentration while <i>R.stolinifer</i> had the low dry weight value of 0.522 gm. The pH values decreased in a fungal cells metabolized after 28 days of incubation. <i>R.stolinifer </i>had the highest pH value of 6.3 after 28 days incubation, but <i>A.niger</i><i> </i>had the lowest PH of 4.6 on Kerosene and there was no significant. The ability of fungi to degrade Kerosene was measured directly by determination the residual Kerosene by FTIR Spectroscopy and indirectly by gravimetric estimation of residual Kerosene left after biodegradation was made by weighing the quantity of Kerosene in a tared flask. The highest percentage loss of Kerosene concentration by the cultures of fungi was 93% by <i>A.niger</i> after 28 day of biodegradation, but the loss of Kerosene concentration in the culture of <i>R.stolinifer</i> reached to 88% after 28 day. Both strains <i>A.niger</i> and <i>R.stolinifer</i> were capable of consuming kerosene as a sole carbon. The data obtained in the present investigation advance our knowledge of kerosene resistance in <i>Aspergillus</i><i> </i><i>niger</i><i> </i>isolated from Iraqi marshes and may make this promising candidates for further investigations regarding their ability to remove kerosene from contaminated environment.},
doi={10.12691/jaem-2-1-7}
publisher={Science and Education Publishing}
}