@article{jaem20261411,
author={{Roseline, NĄ¯DRI Ahou and Alphonse, ALUI Konan and Philom¨¨ne, KOKORA Aya and Yves-Magloire, ANGORATCHI Marius Ebaley and Prisca, ANGOUA Amanahan Mauricette and Ruth, TOURE Kakoumani Lama and Fatoumata, COULIBALY Yele and Detto, KARAMOKO and Aboya, MOROH Jean-Luc},
title={Nitrogen-fixing Potential of Bacillus Strains Isolated from Soils and Roots Across Different Levels of Anthropization in C?te dĄ¯Ivoire},
journal={Journal of Applied & Environmental Microbiology},
volume={14},
number={1},
pages={1--8},
year={2026},
url={https://pubs.sciepub.com/jaem/14/1/1},
issn={2373-6712},
abstract={In the context of declining soil fertility associated with agricultural intensification in C?te dĄ¯Ivoire, the use of plant growth promoting rhizobacteria (PGPR) represents a sustainable alternative to nitrogen-based inputs. This study assessed the atmospheric nitrogen-fixing potential of cultivable bacteria isolated from rhizospheric soils and roots (endophytes) collected along a gradient of anthropization. A total of sixty-five (65) samples were collected from anthropized areas (maize and cassava fields) and low-anthropized areas (Banco Forest, Haut Bandama Reserve, and Lataha). Following isolation on nutrient agar and Pseudomonas Agar, 234 isolates were purified (78.63% rhizospheric; 21.37% endophytic). Nitrogen-fixing capacity was screened <i>in vitro</i> using nitrogen-free peptone water, with colorimetric quantification of ammonia production. Cultivable bacterial densities were generally higher in soils than in roots and tended to increase in low-anthropized sites. Among the isolates, 96.15% produced ammonia, with 14 isolates showing high performance after 72 h of incubation. The concentrations produced by these bacteria ranged from 18.60 x 10<SUP>-3</SUP> to 11.05 x 10<SUP>-3</SUP> mg/ml. Three sporulating isolates were selected and identified using the API 50 CHB system as <i>Bacillus cereus</i> (88.6%), <i>Bacillus coagulans</i> (70.5%), and <i>Brevibacillus laterosporus</i> (83%). Ammonia assimilation increased linearly with bacterial abundance, and strain-specific efficiency differed significantly, with <i>Brevibacillus laterosporus</i> exhibiting the highest performance. These results highlight the potential of sporulating bacilli as promising biofertilizer candidates.},
doi={10.12691/jaem-14-1-1}
publisher={Science and Education Publishing}
}