@article{ijebb2015332,
author={{U.M., Obiakalaije and O.A., Makinde and E.R., Amakoromo},
title={Bioremediation of Crude Oil Polluted Soil Using Animal Waste},
journal={International Journal of Environmental Bioremediation & Biodegradation},
volume={3},
number={3},
pages={79--85},
year={2015},
url={http://pubs.sciepub.com/ijebb/3/3/2},
issn={2333-8636},
abstract={Crude oil contaminated soil from Isaka mangrove in Okirika local government area of Rivers state was treated with three different organic wastes (goat manure, poultry droppings and cow dung), for a period of 28 days. The four treatment samples were tilled twice a week and watered with 50 ml of distilled water weekly. There was a general increase in microbial count for all the treatments with the amended samples having a higher microbial count. The total heterotrophic bacterial count for the A, B, C and D treatment options increased from 2.85x10<SUP>5</SUP>-1.95x10<SUP>6</SUP>cfu/g, 3.02x10<SUP>5</SUP>-3.09x10<SUP>6</SUP>cfu/g, 2.75x10<SUP>5</SUP>-2.69x10<SUP>6</SUP>cfu/g and 2.88x10<SUP>5</SUP>-2.51x10<SUP>6</SUP>cfu/g respectively. The hydrocarbon utilizing bacterial count for the A, B, C, and D treatment options increased from 2.51x10<SUP>5</SUP>-1.74x10<SUP>6</SUP>cfu/g, 2.85x10<SUP>5</SUP>-2.95x10<SUP>6</SUP>cfu/g, 2.63x10<SUP>5</SUP>-2.51x10<SUP>6</SUP>cfu/g and 2.51x10<SUP>5</SUP>-2.29x10<SUP>6</SUP>cfu/g respectively. There was a progressive increase in total heterotrophic fungal count, with B treatment option showing the highest increase at 3.02x10<SUP>6</SUP>cfu/g. The hydrocarbon utilizing fungal count for the A, B, C and D treatment options increased from 1.05x10<SUP>5</SUP>-1.26x10<SUP>6</SUP>cfu/g, 1.10x10<SUP>5</SUP>-2.24x10<SUP>6</SUP>cfu/g, 1.12x10<SUP>5</SUP>-2.09x10<SUP>6</SUP>cfu/g, and 1.10x10<SUP>5</SUP>-1.99x10<SUP>6</SUP>cfu/g respectively. By day 28, the percentage loss of biodegradation of total petroleum hydrocarbon for the B, C, D and A treatment options as measured with GC-FID were 87.1%, 76.6%, 70.7%, and 32.1%, respectively. Ten hydrocarbon utilizing bacterial isolates identified were <i>E</i><i>scherichia</i><i>coli</i> ., <i>Citrobacter</i> sp., <i>Bacillus </i>sp., <i>Micrococcus</i> sp., <i>Pseudomonas </i>sp., <i>Flavobacterium</i> sp., <i>Alicagenes</i> sp., <i>Corynebacterium</i> sp., <i>Arthrobacter</i> sp., <i>Aeromonas</i> sp., and seven hydrocarbon utilizing fungal isolates obtained were <i>Aspergillus</i> sp., <i>Candida </i>sp., <i>Fusarium</i> sp., <i>Mucor</i> sp., <i>Penicillum</i> sp., <i>Rhodotorula</i> sp., and <i>Rhizopus</i> sp. The results of this study indicated that nutrient amendment can enhance the rate of biodegradation of crude oil polluted soil.},
doi={10.12691/ijebb-3-3-2}
publisher={Science and Education Publishing}
}