Science and Education Publishing International Journal of Environmental Bioremediation & Biodegradation 2 4 2014 07 03 Exploited Application of Lactobacillus in Microbial Degradation and Decolorization of Acid Orange 160 166 EN Maulin P Shah Industrial Waste Water Research Laboratory, Division of Applied & Environmental Microbiology, Enviro Technology Limited, GIDC, Ankleshwar, Gujarat, India IJEBB2014243 10.12691/ijebb-2-4-3 2014 04 14 2014 06 28 2014 07 03 A bacterium identified as Lactobacillus was isolated from acclimated sludge from a dyeing wastewater treatment plant. This strain rapidly decolorized Reactive orange (RO) at 0.1% (w/v) concentration of both sucrose and peptone supplemented in Mineral Salt Medium (MSM) under static conditions at a temperature of 30°C with in 24 h with an initial dye concentration of 100 mg/L. The organism exhibited a remarkable color removal capability (95%) even at a high concentration of 1000 mg/L (RO16) dye within 24 h. The biodegradation products were analyzed by, FTIR spectroscopy and LC-MS analysis. The LC-MS analysis indicated the presence of 1-amino- 1-napthalene sulphonic acid in degraded product of the dye.