eng Science and Education Publishing International Journal of Environmental Bioremediation & Biodegradation 2014-06-16 2 4 146 150 10.12691/ijebb-2-4-1 IJEBB2014241 article Sheela. B sheela_bethapudi@yahoo.com 1 khasim beebi. S 2 Yellaji rao.O 3 Department of biotechnology, Gitam institute of science, GITAM University, Visakhapatnam Department of biotechnology, Gitam institute of Technology, Gitam University, Visakhapatnam Environment and safety, synergies and castings limited, Visakhapatnam Urbanization in the world increased the pollution levels in the environment making it for worsen life. General awareness about pollution is increasing today. To control pollution levels, biological methods are eco-safe and economical. In this study a wild strain of Bacillus species was isolated and optimized for bioremediation studies of ammonia in flask cultures. Strategy was tried to develop for ammonia bioremediation in a single stage aerobic nitrification and denitrification. The results reveal that the optimum pH for the isolated Bacillus species was 7 and optimum temperature was 30°C. At optimum pH and temperature this organism was able to remove 99.2% of ammonia in flask cultures supplemented with 5g/L of ammonium sulphate. The biomass obtained after bioremediation studies was 0.3g/L which is very low. The study on ammonia toxicity revealed that toxicity was due to pH and osmolarity. http://pubs.sciepub.com/ijebb/2/4/1/ijebb-2-4-1.pdf ammonia sewage water Bacillus species growth parameters acclimatization bioremediation