Science and Education Publishing International Journal of Environmental Bioremediation & Biodegradation 2 2 2014 04 13 An Application of Bioaugmentation Strategy to Decolorize & Degrade Reactive Black Dye by Pseudomonas spp 50 54 EN Maulin P Shah Industrial Waste Water Research Laboratory Applied & Environmental Microbiology Lab, Enviro Technology Limited (CETP) IJEBB2014221 10.12691/ijebb-2-2-1 2014 04 08 2014 04 11 2014 04 13 Pseudomonas species was isolated from the textile effluent polluted soil in Ankleshwar, Gujart, India and identified based on Biochemical and 16S RNA Sequence. The present study was carried out in an attempt to decolorize a commonly used yet tougher dye to decolorize, Reactive Black. The decolorization percentage was calculated from UV-Vis spectrophotometric analysis. Dye decolorization was probably due to the biotransformation and depended upon the biomass. Replacement of nutrient broth with minimal media did not show any decolorization property. The decolorization was confirmed by studying the spectral analysis of the dye. 16S rRNA partial gene sequencing (772 bp) of isolate Pseudomonas was also performed and a phylogenetic tree was prepared. Decolorization was optimized and found to be up to 95% at 7 pH, 40°C under static and non-aerated condition.