Science and Education Publishing International Journal of Environmental Bioremediation & Biodegradation 1 1 2013 09 02 Microbial Degradation and Decolorization of Reactive Orange Dye by Strain of Pseudomonas Spp 1 5 EN Maulin P Shah Applied & Environmental Microbiology Lab, Enviro Technology Limited (CETP), Gujarat, India Kavita A Patel Sunu S Nair A M Darji IJEBB2013111 10.12691/ijebb-1-1-1 2013 08 23 2013 08 31 2013 09 02 Azo dye Reactive orange was selected for decolorization and degradation studies by Pseudomonas spp. Optimization of parameters for dye decolorization were studied under static anoxic condition. Under optimized condition decolorization of Reactive orange by Pseudomonas spp was found to be 98% at 50mg/L within five hours in static anoxic condition. The optimum pH and temperature for the decolorization was 8.0 & 37 ˇăC respectively. The biodegradation was monitored by FTIR analysis. The results suggest that the isolated organism Pseudomonas spp as a useful tool to treat wastewater containing reactive dyes.