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Effect of Laser Conditioning and Different Adhesive Strategies on Bond Strength of a Bulk Fill and a Microhybrid Resin Composite

Zumrut Ceren Ozduman*, Duygu Tuncer, Çiğdem Çelik, Neslihan Arhun

Başkent University, Faculty of Dentistry, Department of Restorative Dentistry, Ankara, Turkey

Abstract: Purpose: To evaluate the effect of laser conditioning and adhesive strategies on bond strength of a bulk fill and a microhybrid resin composite. Methods: 96 human posterior teeth’s buccal and lingual surfaces were ground flat to expose dentin surfaces with standard smear layer (N=192). The specimens were divided into 16 experimental groups according to (1) Laser-conditioning (Laser-etched group/control group); (2) Adhesive strategies (etch&rinse, Adper Single Bond2(SB2), two step self-etch ClearfilSE Bond(CSE), multi-mode adhesive All-Bond Universal with self-etching technique(ABU-SE), or etch&rinse technique(ABU-ER); (3) Restorative materials (Z250/SDR). All the restorative applications were applied according to the manufacturer’s directions. The specimens were stored in 100% humidity for 24 hours. The specimens were placed on a shear bond test machine at a crosshead speed of 1 mm/min. The values were recorded at the time of failure of composite resins. The bond strength values for shear forces were reported in MPa and derived by dividing the imposed force at the time of fracture by the bonded area. The failed samples were examined under a streomicroscope at X20 magnification to determine the mode of failure. The data were statistically analyzed with three-way ANOVA and multiple comparisons were made by Bon Ferroni test at 5% significance level (p<0.05). Results: SDR and Z250 yielded statistically significant difference when bonded with ABU-ER without laser conditioning (p=0.008). The predominant mode of failure was mixed type. Conclusion: Laser conditioning did not affect shear bond strength values of a bulk fill and a microhybrid resin composites regardless of adhesive system.

Keywords: bond strength, adhesive systems, laser, bulk fill resin composites

Received June 29, 2018; Revised August 02, 2018; Accepted August 14, 2018

Citation: Zumrut Ceren Ozduman, Duygu Tuncer, Çiğdem Çelik, Neslihan Arhun, Effect of Laser Conditioning and Different Adhesive Strategies on Bond Strength of a Bulk Fill and a Microhybrid Resin Composite. International Journal of Dental Sciences and Research, 6 (5). 124-128. January 2018. doi:10.12691/ijdsr-6-5-3

1. Introduction

The search for faster and easier restorative procedures while maintaining adhesive efficiency and adequate bond strength is the backbone of progressive dental technologies. Recently the manufacturers have introduced the bulk fill resin composites which can be placed in bulks ranging from 4 to 6mm, instead of the conventional 2 mm incremental technique [1]. Placement of bulk fill resin composites involve less application steps by reducing the time-consuming total number of layers and polymerization period compared to the conventional resin composites.

Contemporary dental adhesives focus on providing effective bond strength values to different dental substrates. [2] Manufacturers developed various adhesive systems, regarding different adhesive strategy and application steps. These adhesive systems offer the clinician the opportunity to consider which adhesive strategy could be used: They are intended to “all-in-one” one-step self-etch adhesive concept and known as “multi-mode” or “universal” adhesive systems [3,4,5,6].

In recent years, many types of laser systems can be used for dental applications like dentin/enamel conditioning or cavity preparation, due to their ability to efficiently remove dental tissues [7,8,9]. Successful removal of dental hard tissues while minimizing thermal damage risk and avoiding smear layer formation may be accomplished by a recently developed hydrokinetic laser named Er, Cr: YSGG laser [10]. Surface pretreatment is one of the particular benefits of Er,Cr:YSGG laser. Er,Cr:YSGG laser treatment is found to be an effective substitute for conventional acid-etching on enamel [11,12]. However, for dentin, some studies [9,13] demonstrated lower bond strengths with diamond bur application compared to the Er,Cr:YSGG laser ablated collagen fibers. Therefore, several studies have concentrated on laser ablation on enamel and dentin conditioning and reported that there were some micro porosities on tooth structures with absence of smear layer [14,15,16,17,18,19,20].

The bond strength is one of the major important factors that determine the success and longevity of the resin based restorations [21,22,23,24]. However, dental literature is limited about the effect of laser conditioning on the bond strength of multimode adhesive systems and bulk fill resin composites.

The purpose of this in vitro study was to evaluate the effect of laser conditioning and different adhesive strategies on the bond strength of bulk fill resin composites to dentin. The null hypothesis tested was bond strength values are not affected by laser-conditioning regardless of adhesive system and resin composite types.

2. Materials and Methods

This study was supported by Başkent University Research Fund and approved by Başkent University Institutional Review Board (Project no: D-KA 16/04). 96 caries free and intact, anonymized human molars were used for the study. All teeth were embedded in self-curing acrylic resin, then, buccal and lingual surfaces were ground flat to expose dentin surfaces and polished with 600-grit silicone carbide paper under running water to obtain standard smear layer (N=192). The specimens were randomly divided into 16 experimental groups according to (1) Laser irradiation (Laser irradiated group/control group); (2) Adhesive strategies (etch&rinse, Adper Single Bond 2 (SB2), two-step self-etch Clearfil SE Bond (CSE), multi-mode adhesive All-Bond Universal with self-etching technique (ABU-SE), or etch&rinse technique (ABU-ER); (3) Restorative materials (Filtek Z250/Surefil SDR Flow) (n=12) (Table 1).

Table 1. Test Groups

Table 2. Materials used in the study

Dentin surfaces were irradiated with Er,Cr:YSGG laser (Waterlase MD, Biolase Technology Inc., CA, USA) with a power of 1 W at a wavelength of 2780 nm and a 20 Hz frequency with a pulse duration of 140 μs under 55% of water and %65 of air cooling. Laser was used in focused mode and the focal distance of the sapphire tip (MZ6, 600 μm in diameter) was 1-2 mm.

All restorative materials were applied according to the manufacturer’s instructions (Table 2).

Following application of the adhesive, a plastic tube (3 mm in diameter and 4 mm in length) was placed on the dentin surface and filled with Filtek Z250 in two consecutive increments of 2 mm and in one 4 mm increment for Surefil SDR Flow, followed by polymerizing each increment with a LED curing device (1000 mW/cm; LEDMAX 550, Benlioglu Dental, Ankara, Turkey) for 20 seconds. The specimens were stored in 100% humidity at 37°C for 24 hours before shear bond strength test measurements.

The specimens were placed on a Universal Testing Machine (Lloyd-LRX; Lloyd Instruments, Fareham, UK) and loaded at a cross-head speed of 1 mm/min. The values were recorded at the time of failure of composite resins. The shear bond strength values were calculated in MPa by dividing the shear load (Newton) at the time of fracture by the bonded area (mm²). The failure modes were evaluated by a stereomicroscope at X20 magnification as adhesive, cohesive or mixed failures.

The results were analyzed using the statistical package Statistical Package for the Social Sciences (SPSS) 14.0.0 for Windows (SPSS Inc., Chicago, IL, USA). The data were statistically evaluated by three-way ANOVA and multiple comparisons were made using Bonferroni test at a significance level 0.05. (p<0.05)

3. Results

The mean shear bond strength values, standard deviations and p values are shown in Table 3. No statistically significant difference was found between the groups evaluated (p>0.05). There was only a statistically significant difference between Surefil SDR and Filtek Z250 groups, bonded with ABU-ER without laser conditioning (p=0.008).

Table 3. Mean, standard deviations and p values of the groups tested

The frequencies of failure mode for all groups are shown in Figure 1. Analysis of bond failure showed a greater incidence of mixed failures for all groups.

Figure 1. Failure modes of the groups tested. ABU SE: All Bond Universal (self-etch); ABU ER: All Bond Universal (etch&rinse); SB2: Adper Single Bond2; CSE: Clearfil SE Bond; L: Laser

4. Discussion

The results of the present study demonstrated no statistically significant difference between the shear bond strength values of groups with or without laser conditioning regardless of adhesive system and resin composite type used, thus the null hypothesis was accepted. The dental literature contains contradictory results about the use of lasers for dentin conditioning. Ramos et al. reported lower micro-tensile bond strength values on laser-treated dentin surfaces [25]. Ansari et al. [9] compared the micro-shear bond strength of resin composite, after bur or laser and acid or laser conditioning. The results demonstrated that additional etching with phosphoric acid after Er,Cr:YSGG laser pretreatment may be necessary to improve the bond strength. On the other hand, in an in-vitro study by Chou et al., the bond strength of the laser-irradiated groups and the acid-etched groups yielded similar results on dentin [26].

According to Bachmann et al., laser irradiation might alter the structure and form of the organic matrix leading to obstruction of the adhesive penetration and expedition collagen degradation [27]. Although laser irradiated dentin surfaces exhibit an etching pattern, additional micro fragment-like structures might impair the adhesion of resin composite materials [28]. The controversial results in the literature may be related to the employed materials and methods like the physical parameters of the laser, resin material types or application of additional acid etching after laser conditioning [9].

Several studies compared different output settings of erbium lasers in terms of bond strength [7,8,9]. Başaran et al. [29] reported that increasing laser output had led to increased SBS values. Besides, lower power laser irradiation does not produce adequate changes on the tooth surface [30]. Thus the output of the laser is of significant importance since adhesion of composite resin restorations has been affected by the laser irradiation [31,32].

According to the data collected in the present study, Surefil SDR exhibited similar bond strength values as the other conventional resin composite Filtek Z250. Ilie et al. [33] evaluated the shear bond strength of bulk fill resin composites. Their results showed that performance of bulk fill resin composites was comparable or better than the nanohybrid resin composites. Contrary to our findings, Surefil SDR showed significantly high bond strength values in their study. However, it is clinically obligatory to cover a micro/nano hybrid resin composite layer owing to the low mechanical properties of Surefil SDR and this additional procedure prolongs the application time [33].

In a previous study, the shear bond strength of bulk fill and nanohybrid resin composites were evaluated and no statistically significant difference was observed between the groups tested. This finding is aligned with the outcomes of our study. The authors of that study also concluded that this finding could be of potential benefit to the clinicians because restoring with bulk fill resin composites are simpler than conventional composites and can be applied more efficiently [34].

‘Universal’ dentin adhesive systems are recently introduced into the market; however, the indications of these systems are still under debate. All-Bond Universal contains small amount of water and ethanol as solvent and the pH is approximately 3.2. Jang et al. reported that very hydrophobic character of All-Bond Universal might enhance its adhesive performance for both application modes. According to their results, Single Bond Plus has demonstrated lower bond strength values than Clearfil SE and All-Bond Universal [35]. In the present study, there was no significant difference between the bond strength values of adhesive systems used. Vermelho et al. [36] compared the multimode adhesives with the self-etch and etch&rinse systems and reported that those adhesives showed good bond strength performance as well as the control groups regarding dentin. They also reported that biphenyl dimethacrilate (BPDM) and 10-MDP in All-Bond Universal, as its functional monomers might be responsible for the similar bond strength values when compared to Clearfil SE Bond.

The SBS testing methodology may be considered as the limitation of the present study. This method had been in use for the last three decades [37,38]. Although, this methodology is less complex and simpler to use than the micro testing counterparts, the discriminative efficiency may not be conclusive [38]. However, the shear bond strength method has been frequently being used to characterize the mechanical properties of the materials.

5. Conclusion

Based on the results of the present study, it can be concluded that laser conditioning did not affect the bond strength values of different types of adhesive systems and resin composites used.

Acknowledgments

The authors would like to thank Prof. Ayse Rüya Yazıcı from Hacettepe University Faculty of Dentistry Dept. of Restorative Dentistry for her kind contribution in laser application and Dr. Sevilay Karahan from Hacettepe University Faculty of Medicine Dept. of Biostatistics for statistical analysis.

Statement of Competing Interests

The authors have no competing interests

List of Abbreviations

SB2: Adper Single Bond2

CSE Clearfil SE Bond

ABU-SE: All-Bond Universal with self-etching technique

ABU-ER: All-Bond Universal etch&rinse technique

L: Laser

References

[1]   | Furness, A., Tadros, M.Y., Looney, S.W., Rueggeberg, F.A. “Effect of bulk/incremental fill on internal gap formation of bulk-fill composites”, J Dent, 42(4). 439-449. 2014.
[2]   | Van Meerbeek, B., Yoshihara, K., Yoshida, Y., Mine, A., De Munck, J., Van Landuyt, K.L. “State of the art of self-etch adhesives”, Dent Mater, 27(1). 17-28. 2011.
[3]   | Wagner, A., Wendler, M., Petschelt, A., Belli, R., Lohbauer, U. “Bonding performance of universal adhesives in different etching modes”, J Dent, 42(7). 800-807. 2014.
[4]   | de Goes, M.F, Shinohara, M.S, Freitas, M.S., “Performance of a new one-step multi-mode adhesive on etched vs non- etched enamel on bond strength and interfacial morphology”, J. Adhes. Dent, 16(3). 243-250. 2014.
[5]   | Hanabusa, M., Mine, A., Kuboki, T., Momoi, Y., Van Ende, A., Van Meerbeek, B., De Munck, J., “Bonding effectiveness of a new ‘multi-mode’ adhesive to enamel and dentine”, J Dent, 40(6). 475-484. 2012.
[6]   | Munoz, M.A., Sezinando, A, Luque-Martinez, I., Szesz, A.L., Reis A., Loguercio, A.D., Bombarda N.H., Perdigão, J., “Influence of a hydrophobic resin coating on the bonding efficacy of three universal adhesives”, J Dent, 42(5). 595-602. 2014.
[7]   | Gutknecht, N., Apel, C., Schäfer, C., Lampert, F., “Microleakage of composite fillings in Er, Cr:YSGG laser prepared class II cavities”, Lasers Surg Med, 28(4). 371-374. 2001.
[8]   | Shahabi S, Ebrahimpour L, Walsh LJ. “Microleakage of composite resin restorations in cervical cavities prepared by Er, Cr:YSGG laser radiation”, Aust Dent J, 53(2). 172-175. 2008.
[9]   | Jaberi Ansari, Z., Fekrazad, R., Feizi, S., Younessian, F., Kalhori, K.A., Gutknecht N. “The effect of an Er, Cr: YSGG laser on the micro‐shear bond strength of composite to the enamel and dentin of human permanent teeth”, Lasers Med Sci, 27(2): 761-765. 2012.
[10]   | Eversole, L.R, Rizoiu, I., Kimmel, A.I. “Pulpal response to cavity preparation by an erbium, chromium: YSGG laser-powered hydrokinetic system”, J Am Dent Assoc, 128(8). 1099-106. 1997.
[11]   | Baygin, O., Korkmaz F.M., Tüzüner, T., Tanriver, M. “The effect of different enamel surface treatments on the microleakage of fissure sealants”, Lasers Med Sci, 27(1):153-160. 2012.
[12]   | Türkmen, C., Sazak-Oveçoğlu, H., Günday, M., Güngör, G., Durkan, M., Oksüz, M. “Shear bond strength of composite bonded with three adhesives to Er,Cr:YSGG laser–prepared enamel”, Quintessence Int, 41(6). 119-24. 2010.
[13]   | Shahabi, S., Chiniforush, N., Bahramian, H., Monzavi, A., Baghalian, A., Kharazifard, M.J. “The effect of erbium family laser on tensile bond strength of composite to dentin in comparison with conventional method”. Lasers Med Sci, 28(1). 139-42. 2013.
[14]   | Hossain M, Nakamura Y, Yamada Y, Kimura Y, Matsumoto N, Matsumoto K. “Effects of Er, Cr:YSGG laser irradiation in human enamel and dentin: ablation and morphological studies” J Clin. Laser Med Surg, 17(4):155-159. 1999.
[15]   | Trajtenberg C.P., Pereira P.N., Powers J.M., “Resin bond strength and micromorphology of human teeth prepared with an erbium: YAG laser”, Am J Dent, 17(5). 331-336. 2004.
[16]   | Aranha, A.C., De Paula, E.C., Gutknecht, N., Marques, M.M., Ramalho, K.M., Apel, C. “Analysis of the interfacial micromorphology of adhesive systems in cavities prepared with Er, Cr: YSGG, Er: YAG laser and bur” Micros. Res. Tech, 70(8). 745-751. 2007.
[17]   | Basaran, G., Ozer, T., Berk, N., Hamamci, O. “Etching enamel for orthodontics with an erbium, chromium:yttrium-scandium-gallium-garnet laser system”, Angle Orthod, 77(1). 117-123. 2007.
[18]   | Basaran, G., Hamamci, N., Akkurt, A. “Shear bond strength of bonding to enamel with different laser irradiation distances”, Lasers Med Sci, 26(2). 14-156. 2011.
[19]   | Esteves-Oliveira, M., Zezell, D.M., Apel, C., Turbino, M.L., Aranha, A.C., Eduardo, C., Gutknecht N. “Bond strength of self-etching primer to bur cut, Er, Cr:YSGG, and Er:YAG lased dental surfaces”, Photomed Laser Surg, 25(5) 37-380. 2007.
[20]   | Zhang, S., Chen, T., Ge, L.H., “Scanning electron microscopy study of cavity preparation in deciduous teeth using the Er:YAG laser with different powers”, Lasers Med Sci, 27(1):141-144. 2012.
[21]   | Yoshikawa, T., Burrow, M.F., Tagami J. A light curing method for improving marginal sealing and cavity wall adaptation of resin composite restorations. Dent Mater, 17(4). 359-366. 2001.
[22]   | Al-Boni R., Raja O.M., “Microleakage evaluation of silorane based composite versus methacrylate based composite”, J. Conserv. Dent, 13(3):152-155. 2010.
[23]   | Yazici, A.R., Yıldırım, Z, Antonson, S.A., Kilinc, E., Koch, D., Antonson, D.E., Dayangaç, B, Ozgünaltay, G. “Comparison of the Er, Cr:YSGG laser with a chemical vapour deposition bur and conventional techniques for cavity preparation: A microleakage study” Lasers Med Sci, 27(1):23-29. 2012.
[24]   | Obeidi, A., Liu, P.R., Ramp, L.C., Beck, P., Gutknecht, N., “Acid‐etch interval and shear bond strength of Er, Cr:YSGG laser prepared enamel and dentin”, Lasers Med Sci,; 25(3). 363-369. 2010.
[25]   | Ramos, T.M., Ramos-Oliveira, T.M., Moretto, S.G., de Freitas, P.M., Esteves-Oliveira, M., de Paula Eduardo, C. “Microtensile bond strength analysis of adhesive systems to Er:YAG and Er. Cr:YSGG laser- treated dentin” Lasers Med Sci, 29(2). 565-73. 2014.
[26]   | Chou, J.C., Chen, C.C., Ding, S.J., “Effect of Er, Cr:YSGG laser parameters on shear bond strength and microstructure of dentine”, Photomed Laser Surg, 27(3). 481-486. 2009.
[27]   | Bachmann, L., Diebolder, R., Hibst, R., Zezell, D.M., “Changes in chemical composition and collagen structure of dentine tissue after erbium laser irradiation” Spectrochimica Acta Part A, Mol Biomol Spectrosc, 61:2634-2639. 2005
[28]   | De Munck, J., Van Landuyt, K., Peumans, M., Poitevin, A., Lambrechts, P., Braem, M., Van Meerbeek, B. “A critical review of the durability of adhesion to tooth tissue: methods and results” J Dent Res, 84(2). 118-132. 2005.
[29]   | Başaran, E.G., Ayna, E., Başaran, G., Beydemir, K. “Influence of different power outputs of erbium, chromium: yttrium-scandium-gallium-garnet laser and acid etching on shear bond strengths of a dual-cure resin cement to enamel”, Lasers Med Sci, 26(1). 13-19. 2011.
[30]   | Sun, X., Ban, J., Sha, X., Wang, W., Jiao, Y., Wang, W., Yang, Y., Wei, J., Shen, L., Chen, J. “Effect of Er,Cr:YSGG Laser at Different Output Powers on the Micromorphology and the Bond Property of Non-Carious Sclerotic Dentin to Resin Composites” PLoS One, 10(11). e0142311. 2015.
[31]   | Visuri SR, Gilbert JL, Wright DD, Wigdor HA, Walsh JT Jr. “Shear strength of composite bonded to Er: YAG laser-prepared dentin”, J Dent Res, 75(1): 599–605. 1996.
[32]   | De Munch, J., Van Meerbeek, B., Yudhira, R., Lambrechts, P., Vanherle, G. “Micro-tensile bond strength of two adhesives to Erbium: YAG-lased vs. bur-cut enamel and dentin” Eur J Oral Sci, 110(4). 322-329. 2002.
[33]   | Ilie, N., Schöner, C., Bücher, K., Hickel, R. “An in-vitro assessment of the shear bond strength of bulk-fill resin composites to permanent and deciduous teeth” J Dent, 42(7):850-855. 2014.
[34]   | Colak, H., Ercan, E., Hamidi, M.M., “Shear bond strength of bulk-fill and nano-restorative materials to dentin” Eur J Dent, 10(1): 40-45. 2016.
[35]   | Jang, J.H., Lee, M.G., Woo, S.U., Lee, C.O., Yi, J.K., Kim, D.S. “Comparative study of the dentin bond strength of a new universal adhesive” Dent. Mater J, 35(4): 606-612. 2016.
[36]   | Vermelho, P.M., Reis, A.F., Ambrosano, G.M., Giannini, M. “Adhesion of multimode adhesives to enamel and dentin after one year of water storage” Clin Oral Investig, 21(5): 1707-1715. 2017.
[37]   | Scherrer, S.S., Cesar, P.F., Swain, M.V. “Direct comparison of the bond strength results of the different test methods: a critical literature review” Dent Mater, 26(2). 78-93. 2010.
[38]   | De Munck, J., Mine, A., Poitevin, A., Van Ende, A., Cardoso, M.V., Van Landuyt, K.L., Peumans, M., Van Meerbeek, B. “Meta-analytical review of parameters involved in dentin bonding” J Dent Res, 91. 351-357. 2012.