eng Science and Education Publishing International Journal of Clinical Nutrition 2014-01-15 2 1 11 17 10.12691/ijcn-2-1-2 IJCN2014212 article Walaa G. Hozayen walaahozayen@hotmail.com 1 Howida S. Abou Seif 2 Susan Amin 3 Biochemistry Department, Faculty of Science, Beni-Suef University Medical Physiology Department, National Research Center, Cairo, Egypt University of Connecticut Health Center, Farmington Avenue Farmington, Connecticut, USA The present study was conducted to evaluate the protective role of rutin and hesperidin on experimental doxorubicin induced hepatotoxicity. Doxorubicin (DXR) administered rats (25 mg / kg; three times intraperitoneally / week for two weeks) were pretreated with rutin, hesperidin, or their mixture (50 mg / kg body weight) three times per week for three weeks. Results showed that DXR caused a marked rise in serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and gamma glutamyl transferase (GGT) activities alongside an increase in serum total bilirubin, α-fetoprotein (AFP) and sialic acid levels Concerning oxidative stress and antioxidant defense system, the depleted hepatic glutathione content of DXR-administered rats was increased above normal levels as a result of pretreatment with rutin, hesperidin and both rutin and hesperidin. However, while elevated lipid peroxidation was noticed in DXR treated rats, pretreatment with rutin, hesperidin or both produced a detectable decrease in the lipid peroxidation level. Taken these data together, it can be concluded that natural plant components such as Rutin and Hesperidin could protect the liver against DXR-induced liver toxicity. http://pubs.sciepub.com/ijcn/2/1/2/ijcn-2-1-2.pdf rutinhesperidinhepatotoxicityoxidative stress