@article{ijcen2014222,
author={{Awooda, Hiba A and Sharara, Gihan M and Mahmoud, Saeed A},
title={Tumor Necrosis Factor-¦Á in Rats Following Transient Focal Cerebral Ischemia Reperfusion and Its Relation to Oxidative Stress},
journal={International Journal of Clinical and Experimental Neurology},
volume={2},
number={2},
pages={24--28},
year={2014},
url={http://pubs.sciepub.com/ijcen/2/2/2},
abstract={<b>Background</b>: The role of TNF-¦Á in ischemic/reperfusion (I/R) is still controversial. The aim of this study was to assess TNF-¦Á in rats subjected to transient cerebral I/R and to correlate their levels with the resulting neurological deficits and oxidative stress biomarkers malondialdehyde and total antioxidant capacity (TAC). <b>Material and</b> <b>Method:</b> Experimental procedures were performed on 30 adult male Wistar rats. Divided into two groups fifteen rats in each, test group subjected to transient focal cerebral I/R by occlusion of the left common carotid artery (CCA) for 30 minutes followed by reperfusion for 24-hours. A control group underwent the surgery at the same neck region without occlusion of the CCA. Neurobehavioral assessments were evaluated. TNF-¦Á was measured using ELISA method. Malondialdehyde and TAC were estimated colorimetry. <b>Results</b>: In the test group TNF-¦Á and Malondialdehyde concentration in both serum and brain tissue were significantly higher than control group (<i>P </i>=0.000). In contrast, the serum and brain tissue levels of TAC in the test group was significantly lower compared to the sham operated rats (<i>P </i>= 0.000). The brain tissue and serum level of TNF-¦Á were correlated negatively with neurological deficit and TAC and positively with Malondialdehyde (<i>P = 0.000</i>). <b>Conclusion: </b>the present study revealed a potential injurious role of TNF-¦Á in rats subjected to cerebral I/R and demonstrated a direct relationship between TNF-¦Á and oxidative stress biomarkers and the consequent neurological deficits.},
doi={10.12691/ijcen-2-2-2}
publisher={Science and Education Publishing}
}