eng Science and Education Publishing International Journal of Celiac Disease 2334-3486 2021-05-18 9 2 77 81 10.12691/ijcd-9-2-2 IJCD2021922 article Mahmoud M. M. Zaky 1 Saly M. E. Toubar 1 Ahmed Samy El-Shafey ahmedsamymmd@gmail.com 2 Botany Department, Faculty of Science, Port-Said University, Port-Said, Egypt Microbiology Section, Faculty of Science, Tanta University, Tanta, Egypt Water pollution is one of the most severe environmental problems that affect public health and can cause diseases as gastrointestinal diseases. Microbial pollution of Lake Manzala was studied in four main localities, Kapoty, Bashtier, Mataryia and Gamil areas which receive a high load of sewage, agricultural and industrial wastes from different sources. A total of 50 bacterial isolates from water and fish were identified using API 20E system, revealed that 20% of these bacterial strains, were related to Escherichia coli (E. coli), which has been subjected to different antibiotics, such as Ampicillin, Penicillin G, Streptomycin, tetracycline, Gentamycin, Chloramphenicol, rifampicin and Cefotaxime. All strains variation of resistance pattern, harboring Plasmid DNA are an indication of risk to human health in the communities around Manzala Lake, from such bacterial pathogens, which live in polluted and stressed environment. http://pubs.sciepub.com/ijcd/9/2/2/ijcd-9-2-2.pdf Microbial pollution Manzala lake antibiotics Plasmid DNA