@article{ijcd2016436,
author={{Primec, Ma?a and Klemenak, Martina and Aloisio, Irene and Gorenjak, Mario and Gioia, Diana Di and Mi?eti?-Turk, Du?anka and Langerholc, Toma?},
title={Faecal Concentrations of Short-chain Fatty Acids and Selected Bacteria in Healthy and Celiac Children},
journal={International Journal of Celiac Disease},
volume={4},
number={3},
pages={95--101},
year={2016},
url={http://pubs.sciepub.com/ijcd/4/3/6},
issn={2334-3486},
abstract={<i>Background</i>: Knowledge about the interplay between diet, microbiota and short-chain fatty acids (SCFAs) so far exists. Moreover, raising evidence suggests their influence on the pathogenesis of the celiac disease (CD). <i>Objective</i>: Our aim was to study and evaluate differences in the composition of selected bacterial groups and SCFAs in faeces of healthy and CD children. <i>Methods</i>: The study included 41 children with CD, 8 newly discovered, not treated children (ND) and 33 children on gluten-free diet for more than 1 year (GFD) and 17 healthy children as a Control group. Bacterial communities and SCFAs in faecal samples were determined by real-time PCR and HPLC analysis, respectively. <i>Results</i>: There were no statistically significant differences between GFD and ND patients. GFD patients compared to Controls had significantly lower <i>Lactobacillus</i> spp. (<i>p</i> = 0.027) and Enterobacteriaceae family group (<i>p</i> = 0.003), but higher propionic acid (<i>p</i> = 0.034). Acetic (<i>p</i> = 0.027) and propionic acid (<i>p</i> = 0.014) were significantly higher in ND patients compared to Controls. <i>Lactobacillus</i> spp. negatively correlated with total SCFAs in the Control and the ND group. In ND and GFD patients, <i>Lactobacillus</i> spp. negatively correlated with <i>Clostridium sensu stricto</i> cluster I. A very strong positive correlation (<i>p</i> = 0.002) between Enterobacteriaceae family and <i>Bacteroides fragilis</i> was found in GFD patients. <i>Conclusions</i>: Changes in microbiota and SCFAs are clearly related to the pathogenesis of CD. As being potential pro-inflammatory agents in CD, acetic and propionic acid may serve as important disease-related markers. Their origin in relation to <i>Lactobacillus</i> and <i>Bifidobacterium</i> is debatable and still need to be further investigated. Enterobacteriaceae family might not be directly addressed to pathogenesis of CD.},
doi={10.12691/ijcd-4-3-6}
publisher={Science and Education Publishing}
}