@article{bse2014211,
author={{Sultana, Mst.Jesmin and Ahmed, Fazle Rabbi Shakil},
title={Biological Characterization of Crude Extract &amp; Pure Compound Isolated from <i>Swertia </i><i>c</i><i>hirata</i> Ham},
journal={Biomedical Science and Engineering},
volume={2},
number={1},
pages={1--4},
year={2014},
url={http://pubs.sciepub.com/bse/2/1/1},
abstract={The fresh stem of the plant <i>Swertia chirata </i>Ham<i> </i>was extracted by rectified spirit. The crude rectified spirit extract was fractionated by using standard chromatographic techniques, on alumina gave several fractions (A, B, C, D, E &amp; F). Fraction D, when subjected to column chromatographic analysis on neutral alumina, yielded a pure compound X-1 m.p. 180¡ãC. X-1 was screened for its antibacterial activities against 12 pathogenic bacteria, 6 Gram positive and 6 Gram negative, by disc diffusion method at a concentration of 200 ¦Ìg/disc. The results obtained were compared with those for a standard antibiotic Kanamycin. X-1 showed significant activity against <i>Bacillus megaterium </i>(13 mm), <i>Bacillus subtilis </i>(11 mm), <i>Salmonella typhi-A </i>(12 mm), <i>Shigella flexeneriae </i>(12 mm) and <i>Klebsiella </i>sp (13 mm) but a little activity against <i>Staphylococcus aureus</i>. The Minimum Inhibitory Concentrations (MIC) of X-1 determined against <i>Bacillus megaterium </i>and <i>Salmonella typhi-A </i>were 128 ¦Ìg/ml and 132 ¦Ìg/ml, respectively when tested in a nutrient broth medium. X-1 also showed significant activity against the brine shrimp (<i>Artemia salina</i>) nauplii (LC<SUB>50</SUB> value of 10 ¦Ìg/ml), in which the mortality rate increased with the increasing concentration of the compound, suggesting a positive correlation between brine shrimp toxicity and cytotoxicity.},
doi={10.12691/bse-2-1-1}
publisher={Science and Education Publishing}
}