eng Science and Education Publishing Biomedicine and Biotechnology 2378-5535 2022-10-22 7 1 6 22 10.12691/bb-7-1-2 BB2022712 article Sabitha Vadakedath tejaswani19@gmail.com 1 Venkataramana Kandi 1 Vikram Godishala 2 Venkata Bharat Kumar Pinnelli 3 Samar Sami Alkafaas 4 Sara Sami EIkafas 5 Prathima Institute of Medical Sciences, Telangana, India Vaagdevi Degree and PG College, Telangana, India Vydehi Institute of Medical Sciences and Research Center, Bengaluru, India Tanta University, Tanta, Egypt Menofia University, Menofia, Egypt Mass spectrometry (MS) is a technique wherein chemical substances are identified in biological and non-biological material. It uses electric and magnetic fields to sort out gaseous ions based on their mass-to-charge ratios. The MS is a technique that uses a mass spectrometer and mass spectrograph as instruments that work by using electric/magnetic fields, and photographic techniques/non-electric means, respectively. The applications of MS include the identification of gases present in the atmosphere. However, applications of MS in medicine assume increased significance owing to the benefit it does in the management of patients, and public health as a whole. Several MS-based clinical diagnostic devices are already available, and many others are in the pipeline. This review presents a comprehensive analysis of the basics of MS, types of MS-based instruments, and their applications. http://pubs.sciepub.com/bb/7/1/2/bb-7-1-2.pdf mass spectrometry chemical substances biological techniques medicine mass spectrograph patients public health diagnostic devices