Science and Education Publishing Biomedicine and Biotechnology 4 1 2017 1 4 Qualitative and Quantitative Analysis of Sinigrin in Different Parts In Vitro and In Vivo of Brassica nigra Plants 19 24 EN Ayyad W. Al Shahawany Department of Biology, Faculty of Education, University of Baghdad, Baghdad, Iraq Zahra N. Al Hattab Samar F. Al Tahhan BB2016414 10.12691/bb-4-1-4 2016 8 26 2016 11 18 2017 1 2 Plants produce a wide range of organic compounds that are not directly involved in their reproduction and normal growth and development. A Laboratory and field study were conducted to optimize the concentration of Sinigrin which produced by Brassica nigra plants. Sinigrin concentrations in plant tissues at various plant parts were monitored using High Performance Liquid Chromatography (HPLC) technique. Concerning, the results of (HPLC) analysis of callus extracts showed that average amount of sinigrin were between 0.1-71.4 (µg g-1). Furthermore, the results of HPLC analysis inducted that sinigrin concentration in seeds, Cotyledon leaves, true leaves and stems of field grown plant were ranged between 6.8-12.75(µg g-1). In conclusion, this study demonstrated that sinigrin is located in all B. nigra plant parts of different concentrations with the highest amount in the mature seeds.