eng Science and Education Publishing Biomedicine and Biotechnology 2017-01-04 4 1 19 24 10.12691/bb-4-1-4 BB2016414 article Ayyad W. Al Shahawany a61_bio@yahoo.com 1 Zahra N. Al Hattab 2 Samar F. Al Tahhan 2 Department of Biology, Faculty of Education, University of Baghdad, Baghdad, Iraq Department of Genetic Engineering, Biotechnology Center, Ministry of Science and Technology, Baghdad, Iraq Plants produce a wide range of organic compounds that are not directly involved in their reproduction and normal growth and development. A Laboratory and field study were conducted to optimize the concentration of Sinigrin which produced by Brassica nigra plants. Sinigrin concentrations in plant tissues at various plant parts were monitored using High Performance Liquid Chromatography (HPLC) technique. Concerning, the results of (HPLC) analysis of callus extracts showed that average amount of sinigrin were between 0.1-71.4 (µg g-1). Furthermore, the results of HPLC analysis inducted that sinigrin concentration in seeds, Cotyledon leaves, true leaves and stems of field grown plant were ranged between 6.8-12.75(µg g-1). In conclusion, this study demonstrated that sinigrin is located in all B. nigra plant parts of different concentrations with the highest amount in the mature seeds. http://pubs.sciepub.com/bb/4/1/4/bb-4-1-4.pdf Brassica nigra callus sinigrin High Performance Liquid Chromatography HPLC