@article{bb2013125,
author={{Adhikari, Suraj Raj and Pant, Bijaya and Pokhrel, Kusum},
title={Antimicrobial Activity of Chemical Compounds from <i>in </i><i>vivo</i> Roots and <i>in</i><i> </i><i>vitro</i> Callus<i> </i>of <i>Withania somnifera</i> (L.) Dunal},
journal={Biomedicine and Biotechnology},
volume={1},
number={2},
pages={21--26},
year={2013},
url={http://pubs.sciepub.com/bb/1/2/5},
abstract={<i>Withania somnifera</i> is an important medicinal plant and a major source of alkaloids and steroids. Large callus was obtained from stem explants in MS medium with additives of 0.5 mgL<SUP>1</SUP> 6-Benzylaminopurine (BAP) + 1.5 mgL<SUP>-1</SUP><SUP> </SUP>¦Á-Napthalene acetic acid (NAA). In phytochemical screening of <i>i</i><i>n vivo</i> and <i>i</i><i>n vitro</i> materials of <i>W.</i><i> somnifera</i>; reducing compounds, cardiac glycosides, basic alkaloids, carotenoids, volatile oils and saponins were detected. Phytochemical compounds found in callus of <i>W.</i><i> somnifera</i> stopped the growth of bacteria <i>Pseudomonas aeruginosa</i>, <i>Proteus vulgaris</i> and <i>Klebsicela pneumonia</i> more effectively than the compounds found in its natural root. Whereas active phytochemical compound found in natural root was more effective to stop the growth of bacteria <i>Escherichia coli,</i> <i>Staphylococcus aureus,</i> <i>Bacillus subtilis</i> and <i>Salmonella typhimurium.</i><i> </i>From this study it can be predicted that specific compounds which is not found in roots of naturally growing plants plant be found in the callus of <i>W. somnifera</i>.},
doi={10.12691/bb-1-2-5}
publisher={Science and Education Publishing}
}