eng Science and Education Publishing American Journal of Zoological Research 2373-6771 2017-05-18 5 2 24 28 10.12691/ajzr-5-2-1 AJZR2017521 article Tahmina Hoq tahmina.hoq@du.ac.bd 1 Asha Rani Das 2 Institute of Education and Research, University of Dhaka, Bangladesh Department of Zoology, Eden Mohila College, Dhaka, Bangladesh Need exists for rapid means of assessing the "health' of water bodies in Bangladesh. This study aims to assess health indicator for aquatic lives. Response to environmental stress extends to tissue from cellular level. Therefore, test animal Heteropneustes fossilis (Singhi) were treated with Zinc (II) Sulphate Heptahydrate (ZnSO4.7H2O) and Psedumonas. These stressors very commonly found in the water bodies of Bangladesh. Treated Heteropneustes fossilis (Singhi) was used to obtain tissue for conducting SDS-PAGE. SDS-PAGE was applied to protein samples to linearize proteins. A dominant band of 70 kDa was found in the gill after chemical exposure and after biological exposure another 70 kDa was expressed in kidney. Presence of 70 kDa suggests that HSP70 can be the ideal stress marker. The present study also indicates that the use of stress protein for biomarker is easy to use and sensitive for assessing water quality. http://pubs.sciepub.com/ajzr/5/2/1/ajzr-5-2-1.pdf HSP70 stress protein biomonitoring environmental stressor