Science and Education Publishing American Journal of Systems and Software 2 3 2014 07 03 Determination of Characteristic Frequency for Identification of Hot Spots in Proteins 81 84 EN Sidhartha Sankar Sahoo Malaya Kumar Hota Department of Electronics and Telecommunication Engineering, Synergy Institute of Engineering & Technology, Dhenkanal 759001, Odisha, India AJSS2014235 10.12691/ajss-2-3-5 2014 06 09 2014 06 27 2014 07 03 Proteins perform their functions by interaction with other molecules known as target. Protein-target interactions are very specific in nature and occur at predefined locations in proteins known as hotspots. For successful protein-target interaction both protein and target must share common spectral component known as characteristic frequency. Characteristic frequency is very importance since it forms basis for protein-target interactions, thus an approach for determination of characteristic frequency in proteins using discrete cosine transform (DCT) is illustrated in this paper. The performance of the proposed method is observed to be better than existing approaches and is illustrated using simulation examples.