@article{ajps2020822,
author={{Chu, Chishih and Hsia, Chiawen and Tsai, Jun-Yu},
title={Pathogenic Fungi and Bacteria from Homogenates and Commercial Beverages of <i>Auricularia polytricha</i>},
journal={American Journal of Pharmacological Sciences},
volume={8},
number={2},
pages={26--30},
year={2020},
url={http://pubs.sciepub.com/ajps/8/2/2},
issn={2327-672X},
abstract={<i>Auricularia polytricha</i> is an important edible and medicinal fungus in Taiwan. The cultivation environment and sterilization of the mushroom cultivation bag before cultivation may affect the fungal species that occur along the fruiting body for <i>Mucor irregularis</i>, <i>M. fusiformis</i>, and <i>Trichoderma longibrachiatum</i> and the stalk, for <i>Hypocrea koningii,</i> <i>Rhodotorula mucilaginosa</i>, and <i>Coprinellus radians</i> from the stalk. The bacterial species associated with <i>A. polytricha</i> were also identified by PCR amplification, PCR-RFLP analysis, sequencing and BLASTn analysis. Bacterial species were identified as <i>Bacillus cereus, Pseudomonas tolaasii, Sphingomonas paucimobilis, Acinetobacter pittii, </i>and<i> Lysinibacillus fusiformi</i>s in the homogenates after Pasteur sterilization. During a flavor test of 11 commercial drinks, the testers reported experiencing diarrhea. Bacterial examination of the drinks found that three samples were contaminated. Five bacterial species were identified as <i>Pantoea agglomerans, Serratia liquefaciens,</i> and <i>Pseudomonas psychrophila </i>in sample 275, <i>Cronobacter sakazakii </i>and <i>Pseudomonas azotoformans</i> in sample 684 and <i>Pseudomonas azotoformans</i> in sample 539, and more than one bacterial species occurred in two samples. In conclusion, pathogenic fungal and bacterial species were obtained from the fruiting bodies, stalks, homogenates, and drinks of <i>A. polytricha</i> after Pasteur sterilization. To prevent foodborne diseases from <i>A. polytricha</i>, sanitation procedures should be enforced during cultivation, processing and post-harvest storage.},
doi={10.12691/ajps-8-2-2}
publisher={Science and Education Publishing}
}