@article{ajps2013154,
author={{Kumar, Amit and Singh, Vijender and Kumar, Praveen},
title={Determination and Validation of Eflornithine Hydrochloride by (Rp-Hplc) Reversed Phase High Performance Liquid Chromatography},
journal={American Journal of Pharmacological Sciences},
volume={1},
number={5},
pages={90--95},
year={2013},
url={http://pubs.sciepub.com/ajps/1/5/4},
abstract={A simple highly sensitive, rapid, precise, accurate and specific RP-HPLC method was developed for quantification of eflornithine hydrochloride (2-difluoromethyl-DL-ornithine; DFMO) in its commercial formulation. In this RP-HPLC method, the separation was performed using BDS Hypersil 5¦Ì C18 (150 X 4.6 mm) column at room temperature by using methanol: 2% of Glacial acetic acid in water (80:20 v/v) as mobile phase. The flow rate was 0.8 mL min<SUP>-1</SUP> with UV detection at 290 nm. The retention time of DFMO was 4.3. Linearity was observed over concentration range of 50-100 ¦Ìg mL<SUP>-1</SUP> for DFMO. The LOD and LOQ were found to be 0.008438 ¦Ìg mL<SUP>-1</SUP> and 0.028126 ¦Ìg mL<SUP>-1</SUP> respectively. The accuracy of the proposed method was determined by recovery studies and found to be 100.5 % for DFMO. The proposed method was validated for ICH guidelines like linearity, limit of detection, accuracy, precision, ruggedness, robustness, and system suitability.},
doi={10.12691/ajps-1-5-4}
publisher={Science and Education Publishing}
}