@article{ajps2013132,
author={AUTHOR = {Kumar, Amit and Singh, Vijender and Kumar, Praveen},
title={A New Spectrophotometric Method for the Determination of Eflornithine Hydrochloride in Parenteral Formulation},
journal={American Journal of Pharmacological Sciences},
volume={1},
number={3},
pages={38--41},
year={2013},
url={http://pubs.sciepub.com/ajps/1/3/2},
issn={2327-6657},
abstract={This work describes the development, validation and stable studies of a new, simple and reliable UV spectroscopy procedure for the analysis of eflornithine hydrochloride. A new rapid, precise, accurate, specific and simple UV Spectrophotometric analytical method was validated to assay eflornithine hydrochloride (DFMO) in parenteral. Measurements were taken at ¦Ëmax 283 using ethanol as the solvent. Beer¡¯s law is obeyed in the concentration ranges 4 ¨C 32¦Ìg mL<SUP>-1</SUP> with a correlation coefficient (r) equal to 1. The values of limit of detection (LOD) were 0.0675¦Ìg mL<SUP>-1</SUP> and limit of quantification (LOQ) were 0.2045¦Ìg mL<SUP>-1</SUP> for DFMO respectively. The value of molar absorptivity and sandell¡¯s sensitivity was 0.3918 ¡Á 104L mol<SUP>-1</SUP> cm<SUP>-1</SUP> and 0.1633¦Ìg cm<SUP>-2</SUP> respectively. The developed method was validated as per International Conference of Harmonization guidelines with respect to specificity, linearity, limit of detection, limit of quantification, accuracy, precision and robustness. The accuracy of the method was 100.23%. The precision demonstrated a relative standard deviation of less than 1%. The results were satisfactory when compared with the literature. The proposed method might be applied in routine quality control in the pharmaceutical industries since it is precise, accurate, simple and economic. Commercial formulation and laboratory prepared mixtures were successfully analyzed using the developed methods.},
doi={10.12691/ajps-1-3-2}
publisher={Science and Education Publishing}
}