Dear Label_Name,

Greetings! I hope you are well.

I am writing on behalf of an international publishing house, Science and Education Publishing, USA (http://www.sciepub.com).

Science and Education Publishing (SciEP) is a multidisciplinary open access publisher of Journals covering the fields of Science, Technology and Medicine. All SciEP publications are subject to high-quality peer review, editorial and production processes and are freely available online.

Welcome to the April, 2013 edition of SciEP newsletter, providing monthly summaries of articles on science & education related topics of interest to scientist and professionals. The mission of our journals is to provide an open access forum for publishing new findings on issues pertinent to scientific and academic field. We also invite you to submit original research papers, review articles, short reports, or case studies are considered for publication in our Multidisciplinary Journals.

American Journal of Public Health Research
DOI: 10.12691/ajphr-1-1-3

The Implementation of a Tele-Homecare System with Service Oriented Architecture and HL7 Message Transmission Standard
Ching-Sung Wang

American Journal of Medical and Biological Research
DOI: 10.12691/ajmbr-1-1-2

The Influence of Ethanol on Pyruvate Kinases Activity in Vivo, in Vitro, in Silico
Lelevich Sergey Vladimirovich, Khrustalev Vladislav Victorovich, Barkovsky Eugene Victorovich, Shedogubova Tatyana Aleksandrovna

American Journal of Medical Sciences and Medicine
DOI: 10.12691/ajmsm-1-1-1

Clinical and Histological Features in Wegener Granulomatosis
Li Cavoli Gioacchino, Passantino Rita, Bono Luisa, Tortorici Calogera, Ferrantelli Angelo, Giammarresi Carlo, Rotolo Ugo

American Journal of Infectious Diseases and Microbiology
DOI: 10.12691/ajidm-1-1-2

The Curse of Lymphatic Filariasis: Would the Continual Use of Diethylcarbamazine Eliminate this Scourge in Papua New Guinea?
Anne Lanham, Lillian Mwanri

We have released issues of some journals on February 2013.
American Journal of Public Health Research
American Journal of Medical Sciences and Medicine
American Journal of Medical and Biological Research
American Journal of Pharmacological Sciences
American Journal of Clinical Medicine Research
American Journal of Nursing Research
American Journal of Cancer Prevention
American Journal of Medicine Studies
American Journal of Infectious Diseases and Microbiology
American Journal of Cardiovascular Disease Research
American Journal of Biomedical Research
American Journal of Mechanical Engineering
American Journal of Microbiological Research
Journal of Preventive Medicine
American Journal of Food and Nutrition
American Journal of Hypertension Research
American Journal of Sports Science and Medicine
Journal of Cancer Research and Treatment
American Journal of Zoological Research
Journal of Biomedical Engineering and Technology
American Journal of Microbiological Research
Biomedical Science and Engineering

We are pleased to announce that submissions are now being accepted for peer review for all the journals across all our subject areas in SciEP.

Please submit your manuscript via our online submission system:
http://www.sciepub.com/author/signin

To unsubscribe from our mailing list, please click on the following link: unsubscribe

I am looking forward to hearing from you.

Kind regards,

David Rain
Email:join@sciepub.com
Science and Education Publishing (http://www.sciepub.com)

Oral Zinc as Adjuvant Therapy for Pediatric Recurrent Pneumonia: A Prospective Study in a Tertiary Care Hospital

Tejesh Malla*, Kalpana K Malla, Brijesh Sathian, Prithuja poudyal, Eva Gauchan, Sahisnuta Basnet

Department of Pediatrics, Manipal College of Medical Sciences, Pokhara, Nepal

Abstract: Zinc is an essential element required for the cell metabolism, including immunity. Therefore Zinc deficiency leads to susceptibility to infections and may affect pulmonary epithelial cell integrity. Many investigators have used zinc supplementation to see it’s effect on various diseases mainly diarrheal diseases and severe pneumonia. This study aims to evaluate the effect of oral Zinc supplementation in treatment and prevention of recurrent pneumonia. 506 Children aged 2 months to 60 months admitted during September 2011 to August 2014 for recurrent pneumonia with no other underlying illness in the Pediatric department of Manipal Teaching Hospital, Pokhara, Nepal were observed. Along with standard antibiotic treatment one group [Group I] received zinc (10 mg for < 6 months and 20 mg for ≥ 6 months for 10 days) and another group [Group II] did not receive Zinc. The primary outcome like resolution of tachypnea, chest in drawing, hypoxia, starting of oral feeds and hospital stay was noted. All cases were followed up every three monthly for one year to see the recurrence of pneumonia. Data was analyzed using SPSS version 16 and p < 0.01 was considered statistically significant. 20.24% of recurrent pneumonia meeting the inclusion criteria was evaluated. Maximum children (65.4%) were of age 2-12 months. Primary as well as secondary outcome was statistically significant (p<0.001) in Group I. The mean ± SD for primary outcome in Group I vs. Group II were –tachypnea (2.96±1.12 vs. 4.67 ± 1.61 days), chest in drawings (1.63 ±0.95 vs. 3.35 ±1.55 days), hypoxia (24.61 ±1.77 vs. 41.67 ± 2.11 hours), starting of oral feed (1.22 ±0.95 vs. 2.55 ± 1.23 days) and hospital stay (4.82 ±1.22 vs. 6.45 ± 1.99 days). In Group I 20/253 (7.9%) children lost follow up and 2/253(0.79%) patient died and in group II 1 (0.39%) died and 30 (11.85%) lost follow up. Recurrence of pneumonia was also significantly less (p<0.001) in zinc recipients. Determinants in two groups by logistic regression showed earlier resolution in zinc recipients with OR at 99% CI 9.654 (5.605, 16.625) for tachypnea, 14.506 (8.064, 26.095) for chest in drawings, 5.860 (3.415, 10.057) for hypoxia, 13.725 (7.270, 25.912) for starting oral feed and 0.075 (0.040, 0.140) for hospital stay. The recurrence of pneumonia was also less in group I with OR 3.348 at 99% CI (2.009, 5.581). Zinc as an adjuvant therapy is effective in treatment and prevention of recurrent pneumonia in children.

Keywords: Adjuvant Therapy Zinc, Pediatrics Recurrent Pneumonia

Received April 08, 2015; Revised April 29, 2015; Accepted June 26, 2015

Citation: Tejesh Malla, Kalpana K Malla, Brijesh Sathian, Prithuja poudyal, Eva Gauchan, Sahisnuta Basnet, Oral Zinc as Adjuvant Therapy for Pediatric Recurrent Pneumonia: A Prospective Study in a Tertiary Care Hospital. American Journal of Public Health Research, 3 (4A). 12-18. January 2015. doi:10.12691/ajphr-3-4A-3

1. Introduction

Zinc is an essential trace element required for maintaining intestinal cells, bone growth, and immune function [1]. Children who are living in low-income settings are often undernourished and zinc deficient. Its deficiency is associated with increased risk of infection, particularly diarrhea and pneumonia [2,3]. Pneumonia is a killer disease and it alone kills over two million deaths per year [4]. A subgroup of children with pneumonia suffer from recurrent pneumonia reported to be 8-9% in different studies [5,6]. The lack of epidemiological studies on recurrent pneumonia from developing countries makes it difficult to plan for its prevention and treatment [7]. Recurrent pneumonia pose a significant challenge to the pediatricians especially when no underlying cause is detected. Therefore, we aimed to see if there are beneficial effects of zinc as an adjuvant therapy in treatment and prevention of recurrent pneumonia.

2. Methodology

2.1. Study Setting

This was a prospective, hospital based case control study conducted at Manipal Teaching Hospital from September 2011 to August 2014. Cohort of cases were from 1st September 2011 – 31st August 2013, they were then followed up for one year for each case till 31st August 2014.

2.2. Sample Size Calculation

In a pilot study done prior to original study for power 99% and α error 1% with 99% level of confidence showed proportion of recurrence in zinc supplemented group to be 0.55 and it was 0.30 in non zinc supplemented group. We estimated total sample size of 182 children in each group and we have enrolled 253 children in each group. We expected loss of cases during follow up.

2.3. Ethical Committee Approval

Approval was obtained from the Ethics committee of the Manipal Teaching Hospital and written consent in the local Nepali language was obtained from the parents of the children before the commencement of the study. For those parents who were unable to read or write, consent statement was verbally read out to them if they agreed their thumb impressions were obtained in the presence of a witness. Study was done according to the latest declaration of Helsinki [8].

2.4. Enrollment of Cases

Children aged 2 months to 60 months with recurrent pneumonia, [pneumonia 2 or more episodes in a single year or 3 or more episodes ever] [9] were enrolled. We considered diagnosis of pneumonia in the following ways:

1. Reported fever, cough and difficulty breathing with a respiratory rate (RR) above the WHO defined age-specific values (RR ≥ 50 breaths per minute for children aged two to 11 months, or RR ≥ 40 breaths per minute for children aged 12 to 59 months [10], with or without chest in drawings.

2. A diagnosis of pneumonia based on chest examination by a physician.

3. A diagnosis of pneumonia based on a chest radiograph (CXR) by using the WHO standardized tool for interpretation of CXR [11]. CXR taken for each child was interpreted by a radiologist blinded to clinical data.

All children were assessed for hypoxemia according to WHO guidelines, SpO2 of 90% [12] by using a pulse oximeter, [Trusatoximeter, GE Healthcare Finland Oy. Kuortaneenkatu 2, FI-0051010, Helsinki, Finland]with a pediatric sensor [Trusatoximeter, TruSignal Integrated Finger sensor GE, TS-F4-MC] Oxygen saturation (SpO2) was recorded after stabilization of the reading for 1 minute. Children with SpO2 of ≤ 90%, oxygen was provided before further evaluation. Nutritional status was assessed by detailed anthropometry. Height: Measured in nearest centimeter by infantometer in children till 2 years and beyond 2 years standing height was measured by using a standard wooden height measuring board. Weight: Measured in nearest 100 grams with the help of standardized weighing machine (Krups Empress Manual Weighing Scale). Grading of nutritional status was done by using classification by Indian Academy of Pediatrics [13].

2.5. Inclusion Criteria

1. Age 2 month to 60 months presenting with recurrent pneumonia with no other underlying illness.

2. Mild and moderate Malnutrition was included.

Figure 1. Enrollment of cases

Figure 2. Recurrent Pneumonia selected – 506 (20.24%)

2.6. Exclusion Criteria

1. Recurrent pneumonia with underlying illnesses like - congenital heart diseases, tracheoesophageal fistula, gastro esophageal reflux, pulmonary tuberculosis, AIDS, severe malnutrition, bronchiectasis, pneumonia in cerebral palsy, suspected immune deficiency or any genetic disorders.

2. Lobar pneumonia.

3. Respiratory distress due to other causes like heart failure or metabolic acidosis, pleural effusion, pneumothorax, Congenital anomalies of the respiratory tract, interstitial lung diseases.

4. Refusal of Consent.

5. Children presenting with recurrent wheezing (defined as 3 episodes over the past 6 months and on treatment with bronchodilators) [14] and who responded to 3 doses of nebulized Salbutamol given 20 minutes apart.

Then the whole population was divided into two groups.

Group I - received zinc with standard antibiotics.

Group II –received only standard antibiotics.

These cases were followed up every three monthly for one year to see the recurrence.

2.6. Dependent Variable/Exposure Variable

Zinc supplementation.

2.7. Independent Variables/Outcome Variables

Definitions of Outcome Measures:

1. Primary outcome: The admitted children were monitored by study physicians at ∼6 hourly intervals from time of admission to clinical recovery until discharge

Primary outcome is defined as follows:

a) Resolution of tachypnea – within 2 days or > 2 days

b) Resolution of chest in drawings – within 2 days or > 2 days

c) Hypoxia – Discontinuation of oxygen – within 48 hours or >48 hours. The absence of hypoxia was confirmed after a second reading taken 30 minutes later.

C) Starting of feed - within 2 days or > 2 days

D) Time to Hospital discharge: 5 days or > 5 days

2. Secondary Outcome: These children were followed up every three monthly for one year after discharge.

1. Re-admission/re-diagnosis with pneumonia – yes / no

2.8. Dose of Zinc Used

Dispersible zinc tablets containing 10 mg of elemental zinc sulfate was used in the study. Children alternately falling under even number were given zinc and odd numbers were not given zinc. As per the guidelines by World Health Organization and UNICEF [15], children till 6 months were given 1 tablet (10 mg) and children more than 6 months were given 2 tablets (20 mg) dissolved in 5 mL of clean water or breast milk. This was given for 10 days. All children were observed for vomiting. For children who vomited within the first 15 minutes, a repeat dose was given.

2.9. Statistical Analysis

Data was collected, tabulated and analyzed using statistical package SPSS 16.0 version. Chi-square test was used to compare the parameters and P value <0.01 was considered statistically significant. Logistic regression analysis was done for primary and secondary outcome after zinc supplementation.

3. Results

Out of 2500 total case admitted the study period, only 506 (20.24%) cases met the criteria and were included in the cohort. [Table 1], out of them 322 (63.63%) were males and 184 (36.36%) females with male: female ratio of 1.75:1.65.4% children belonged to age 2-12 months. (Table 1).

Table 1. Sample Variables

The baseline demographic and clinical variables like age, gender, gestation age at birth, Ethnic group, mode of delivery, birth weight, immunization, socioeconomic status and nutritional status were similar in the two groups with no statistical differences (Table 2).

Table 2. Comparison of Sample Variables and Risk Factors for Pneumonia in Two Groups

Outcome of Pneumonia with Zinc Supplementation:

Time to resolution of pneumonia – tachypnea, chest in drawings, hypoxia, nil per oral days and hospital stay was significantly (p<0.001) shorter in Group I [Table 3]. The mean ± SD for resolution of pneumonia in Group I Vs Group II were - tachypnea (2.96±1.128 vs. 4.67± 1.613 days), chest in drawings (1.632±0.952 Vs 3.359±1.558 days), hypoxia (24.616±1.778 vs. 41.675±2.11 hours), starting of oral feed (1.217±0.957 vs. 2.553±1.238 days) [Table 3].

Table 3. Early Outcome Variables with Mean ±SD Of Pneumonia in Two Groups

In Group I 20/253 (7.9%) children were lost during follow up and 2/253 (0.79%) patient died and in group II 1 (0.39%) died and 30 (11.85%) were lost during follow up period. Hence, recurrence of pneumonia was assessed in 232 (506-22) /45.8% cases in Group I and 222 (506-31) /43.87% cases in Group II. This was also significantly less (p<0.001) in zinc recipients. [Table 4]

Table 4. Secondary outcome variable of pneumonia in two groups

Determinants in Two Groups by Logistic Regression:

There was earlier resolution of respiratory distress in zinc recipients with OR at 99% CI was 9.654 (5.605, 16.625) for tachypnea, 14.506 (8.064, 26,095) for chest in drawings, 5.860 (3.415, 10.057)for hypoxia, 13.725 (7.270, 25.912) for oral feed, 0.075 (0.040, 0.140) for hospital stay respectively. The recurrence of pneumonia was also less in group I with OR 3.348 at 99% CI (2.009, 5.581) [Table 5].

Table 5. Relation of Variables in Zinc and Non Zinc Groups

4. Discussion

There exists conflicting information regarding the efficacy of zinc as an adjunct to treatment for pneumonia in children. Results from Bangladesh [16] and Kolkata, India [17], showed that zinc supplemented children had a shorter duration of recovery from severe pneumonia. However, a trial conducted in South India found zinc to have no effect on recovery status [18]. Out of two studies done in Nepal, one showed no beneficial effect [19] and other showed marginal benefit [14] on short term clinical recovery of severe pneumonia. Unlike our study, all these above mentioned studies were done in severe pneumonia with no follow ups. This study has evaluated the effect of zinc in primary (short term clinical recovery) as well as secondary (recurrence of pneumonia) outcomes of recurrent pneumonia and found a significant beneficial role of zinc.

Severe pneumonia includes a wide spectrum of causes and does not define the specific etiology [20], hence this may be the reason why cases have responded differently to zinc administration in different studies. We evaluated a specific etiology that was bronchopneumonia and have excluded lobar or interstitial pneumonia and maybe Zinc has beneficial role in bronchopneumonia. The differences in study population and methods between studies may also have affected, thus the heterogeneity in the results. Another reason for the difference in results may be differences in the prevalence of zinc deficiency in the different study populations. Therefore a serum zinc levels would have given additional information but unfortunately serum zinc level was not done in any of the studies mentioned.

Recurrent wheezing (28.4%) in this study, which responded to Salbutamol were excluded from the study which decreased the chances of overlapping of diseased conditions. Compared to other studies [5,6], this study showed a high incidence of recurrent pneumonia which was 20.4%. The reason for this may be that mild and moderate malnutrition was included in this study where child maybe zinc deficient, hence immune deficiency leading to increased incidence of recurrent pneumonia.

Recurrent pneumonia was more prevalent in age group of 4-12 months (65.5%). This is the time when children are gradually weaned off from breast milk and additional supplementary food is added. Plus by this is time they grow and the nutritional requirement is more hence they may be deficient of many micronutrients including zinc.

4.1. Zinc Supplementation

Severe zinc deficiency depresses immune function [21], and even mild to moderate degrees of zinc deficiency can impair macrophage and neutrophil functions, natural killer cell activity, and complement activity [22]. These alterations in immune function might explain why low zinc status has been associated with increased susceptibility to pneumonia and other infections in children in developing countries [23,24]. The World Health Organization and UNICEF now recommend short-term zinc supplementation (20 mg of zinc per day, or 10 mg for infants under 6 months, for 10–14 days) to treat acute childhood diarrhea [15]. But there is no universal recommendation for zinc supplementation in pneumonia. As mentioned above many researches have been done in severe pneumonia with mixed results. The dose of zinc used in this study was same as per the WHO recommendation for diarrhea cases and was given for only 10 days.

4.2. Primary Outcome after Zinc Supplementation

Primary outcome in this study was evaluated on the basis of resolution of tachypnea, hypoxia, improvement on chest in drawings, starting of oral feeds, hospital stay. All these parameters showed statistically significant results. Similar findings were also noted by Ehsan Valavi et al [25]. Shorter duration of recovery with similar parameters in severe pneumonia was also observed in other studies [16,17] but again another study showed no effect of Zinc on the recovery status [18]. Yet in another study time for resolution of respiratory distress, tachypnea, dangers signs and hypoxia were similar in two groups but duration of hospital stay was shorter by 9 hrs. in the zinc group [26]. In this study also hospital stay was significantly shorter in zinc supplemented group. Beneficial effect of zinc on hospital stay can reduce the financial burden and sufferings of the patient so also the hospital load will be less as pneumonia is the most common cause in less than 5 years old for admission in the Pediatric Ward of the hospitals.

4.3. Secondary outcome after Zinc Supplementation

Recurrence of pneumonia episodes was significantly less in zinc supplemented group. Beneficial effect of Zinc for reduction in the incidence and prevalence of pneumonia was also reported in a Cochrane database of Systematic reviews in 2010 [27]. Zinc supplemented children had an OR of 0.59 (95% CI 0.41 to 0.83) for pneumonia [1] and in this study the OR was 3.348 (99% CI 2.009 to 5.581).

4.4. Limitations

This being a hospital based study, inferences cannot be generalized. There was no facility in the laboratory to measure zinc level of individual patients . If this could be done, more accurate information would have been obtained. During follow up period 53 cases were lost which might have an effect on the accuracy of the outcome result.

5. Conclusion

Zinc supplementation is beneficial in treatment as well as prevention of recurrent pneumonia. Possibly children having recurrent pneumonia have deficiency in zinc. Appropriate facilities in the laboratory for measurement of zinc levels should be made available. Other possible approaches and remedial measures should include food fortification, dietary diversification, and inclusion of zinc rich vegetables in the food.

List of Abbreviations

SD: Standard deviation

SPSS: Statistical Package for the Social Sciences

OR: Odds Ratio

AIDS: Acquired Immunodeficiency Syndrome

WHO: World Health Organization

RR: Respiratory Rate

CXR: X-ray Chest

SpO2: Peripheral capillary oxygen saturation

VSD: Ventricular septal defect

UNICEF: United Nations Children's Fund

>: More than

>: Less than

≤: Equal and less than

≥: Equal and more than

What this Study Adds

Maximum studies with zinc supplementation have been done in cases of severe pneumonia but this study was done in recurrent pneumonia. This study also gives information on beneficial effect of zinc supplementation in primary as well as secondary outcome of recurrent pneumonia.

Authors’ Contributions

TM responsible for article concept, designing, data collection and analysis, literature review and write up of manuscript. KM responsible for article concept, designing, critical analysis of manuscript writing. BS responsible for statistical analysis and write up of manuscript. PP, EG and SB responsible for literature review and revision of the manuscript. All the authors approved the final document.

Acknowledgements

We thank mothers and the children who participated in this study. We also express our gratitude to Professor and Head of department Dr. K Seshagiri Rao for granting us permission to do the study. We are also thankful to Dr. Isha Bhandari for her contribution in collecting data. We give a special thanks to Dr. Subita Lalchan, Lecturer, Radio diagnosis department for her contribution in interpreting the chest X-ray throughout the study period.

Declaration of Conflicting Interests

The authors declare that there is no potential conflicts of interest with respect to the research, authorship and /or publication of this article.

Funding

The authors received no financial support for the research, authorship and/or publication of this article.

References

[1]   | Bhutta ZA, Black RE, Brown KH, Gardner JM, Gore S,Hidayat A et al. Prevention of diarrhea and pneumonia by zinc supplementation in children in developing countries: Pooled analysis of randomized controlled trials. Zinc Investigators’ Collaborative Group. Journal of Pediatrics 1999; 135 (6): 689-697.
[2]   | Aggarwal R, Sentz J, Miller MA. Role of zinc administration in prevention of childhood diarrhea and respiratory illnesses: a meta-analysis. Pediatrics. 2007; 119 (6): 1120-1130.
[3]   | Shakur MS et al. Serum and hair zinc in severely malnourished Bangladeshi children associated with or without acute lower respiratory infection. Indian Journal of Pediatrics. 2009; 76 (6): 609-614.
[4]   | Wardlaw TM et al. Pneumonia: the forgotten killer of children. Geneva, World Health Organization, 2006.
[5]   | Khaled S, Sherif AM, Kotb AM. Recurrent/Persistent Pneumonia among Children in Upper Egypt. Mediterr J Hematol Infect Dis. 2014; 6 (1): e2014024.
[6]   | Owayed A, Campbell DM, Wang EEL. Underlying Causes of Recurrent Pneumonia in Children. Arch Pediatr Adolesc Med. 2000; 154 (2): 190-194.
[7]   | Ozdemir O, Sari S, Bakirtas A, Zorlu P, Ertan U. Underlying diseases of recurrent pneumonia in Turkish children. Turk J Med Sci. 2010; 40 (1): 25-30.
[8]   | WMA Declaration of Helsinki-Ethical Principles for Medical Research Involving Human Subjects [Internet]. 2013. Available from: http://www.wma.net/en/30publications/10policies/b3/index.html
[9]   | Wald E. Recurrent and non-resolving pneumonia in children. Semin Respir Infect. 1993; 846-58.
[10]   | World Health Organization: Acute respiratory infections in children. Case management in small hospitals in developing countries 1990, Geneva: WHO/ARI/90.5990.
[11]   | World Health Organization Pneumonia Vaccine Trial Investigator’s Group. Standardization of Interpretation of Chest Radiographs for the Diagnosis of Pneumoniain Children. WHO/ V&B/ 01.35. Geneva, Switzerland: World Health Organization; 2001.
[12]   | World Health Organization. Pocket Book of Hospital Care for Children: Guidelines for the Management of Common Illnesses With Limited Resources. Geneva, Switzerland: World Health Organization; 2005.
[13]   | GhaiOP, GuptaP, PaulVK. Ghai Essential Pediatrics: Nutrition and macronutrient disorders In 6th ed. 2008; 93-118.
[14]   | Basnet S, Shrestha PS, Sharma A, Mathisen M, Prasai R, Bhandari N et al. A Randomized Controlled Trial of Zinc as Adjuvant Therapy for Severe Pneumonia in Young Children. PEDIATRICS. 2012; 129 (4): 701-708.
[15]   | World Health Organization and United Nations Children Fund. Clinical management of acute diarrhoea. WHO/UNICEF Joint Statement, August, 2004.
[16]   | Brooks WA, Yunus M, Santosham M, Wahed MA, Nahar K, Yeasmin S, Black RE. Zinc for severe pneumonia in very young children: double-blind placebo-controlled trial. Lancet. 2004; 363 (9422): 1683-8.
[17]   | Mahalanabis D, Lahiri M, Paul D, Gupta S, Gupta A, Wahed MA, Khaled MA. Randomized, double-blind, placebo-controlled clinical trial of the efficacy of treatment with zinc or vitamin A in infants and young children with severe acute lower respiratory infection. Am J ClinNutr. 2004; 79 (3): 430-6.
[18]   | Bose A, Coles CL, Gunavathi, John H, Moses P, Raghupathy P, Kirubakaran C, Black RE, Brooks WA, Santosham M. Efficacy of zinc in the treatment of severe pneumonia in hospitalized children <2 y old. Am J ClinNutr. 2006; 83 (5): 1089-96.
[19]   | Shah GS, Dutta AK, Shah D, MishraOP. Role of zinc in severe neumonia: a randomized double bind placebo controlled study. Italian Journal of Pediatrics 2012; 38: 36
[20]   | Puumalainen T, Quiambao B, Abucejo-Ladesma E, et al; ARIVAC Research Consortium. Clinical case review: a method to improve identification of true clinical and radiographic pneumonia in children meeting the World Health Organization definition for pneumonia. BMC Infect Dis. 2008; 8: 95.
[21]   | Shankar AH, Prasad AS. Zinc and immune function: the biological basis of altered resistance to infection. Am J ClinNutr 1998; 68: 447S-63S.
[22]   | Wintergerst ES, Maggini S, Hornig DH. Contribution of selected vitamins and trace elements to immune function. Ann NutrMetab 2007; 51: 301-23.
[23]   | Bahl R, Bhandari N, Hambidge KM, Bhan MK. Plasma zinc as a predictor of diarrheal and respiratory morbidity in children in an urban slum setting. Am J ClinNutr 1998; 68 (2 Suppl): 414S-7S.
[24]   | Black RE. Zinc deficiency, infectious disease and mortality in the developing world. J Nutr 2003; 133: 1485S-9S.
[25]   | Valavi E, Hakimzadeh M, Shamsizadeh A, Aminzadeh M, Alghasi A. The Efficacy of Zinc Supplementation on Outcome of Children with Severe Pneumonia.A Randomized Double-blind Placebo-controlled Clinical Trial. The Indian Journal of Pediatrics. 2011; 78 (9); 1079-1084.
[26]   | Bansal A, Parmar VR, Basu S, Kaur J, Jain S,Saha A,Chawla D. Zinc Supplementation in Severe Acute Lower Respiratory Tract Infection in Children: A Triple-Blind Randomized Placebo Controlled Trial.The Indian Journal of Pediatrics. 2011; 78 (1): 33-37.
[27]   | Lassi ZS, Haider BA, Bhutta ZA. Zinc supplementation for the prevention of pneumonia in children aged 2 months to 59 months. Cochrane Database of Systematic Reviews 2010; (12): CD005978.