@article{ajmsm2016451,
author={{AL-Salihi, Siham Sh. and Al-Jubouri, Ali S. and Albayati, Asa'ad F. and Mahmood, Yusra AR.},
title={Enterotoxin Detection by PCR in <i>Klebsiella pneumoniae</i> Isolated from Diarrheal Cases among Children in Kirkuk City - Iraq},
journal={American Journal of Medical Sciences and Medicine},
volume={4},
number={5},
pages={92--96},
year={2016},
url={http://pubs.sciepub.com/ajmsm/4/5/1},
issn={2327-6657},
abstract={The presence of genes encoding the heat stable (ST) and heat labile (LT) enterotoxins were detected in <i>Klebsiella spp.</i> using the PCR method with pairs of specific primers designed for the <i>sta</i>, <i>stb</i>, and <i>lth</i> genes described for diarrheagenic <i>Escherichia coli</i>. It was found that the plasmid DNA of the 8 (40%) of the <i>20 Klebsiella pneumoniae</i> isolates isolated from diarrhea cases in children contained the enterotoxin-encoding genes (<i>sta</i>, <i>stb</i>, <i>lth</i>), <i>sta</i> gene was the most frequently in the strains 7 isolates with (35%), <i>stb</i> genes 2 isolates with (10%) and <i>lth</i> 1 isolates with (5%). Of which (1) <i>K. pneumoniae </i>isolate had plasmid DNA included all three genes and 12 did not have any of the genes. None of the strains of <i>K. oxytoca</i>, <i>K. terrigena</i> and <i>K. ornithinolytica</i>, isolated from children with diarrhea, had genes encoding the tested enterotoxins.},
doi={10.12691/ajmsm-4-5-1}
publisher={Science and Education Publishing}
}