@article{ajmr2021935,
author={{Ouattara, Yakoura Karidja and Kouam¨¦-Sina, Sylvie Mireille and Coulibaly, Kalpy Julien and Casimir, Bl¨¦ Yatanan and Beni, Como¨¦ Koffi Donatien and Sabine, Vakou N¡¯dri and Tour¨¦, Andr¨¦ Offianan and Dadi¨¦, Adj¨¦hi},
title={Detection of a Gene Involved in Fumonisin Production in <i>F</i><i>usarium </i>Strains Isolated from <i>Moringa</i><i> </i><i>(</i><i>Moringa</i><i> </i><i>oliefera</i>) Sold in the Markets of Abidjan (C?te d'Ivoire)},
journal={American Journal of Microbiological Research},
volume={9},
number={3},
pages={96--102},
year={2021},
url={http://pubs.sciepub.com/ajmr/9/3/5},
issn={2328-4137},
abstract={Fumonisins produced by <i>Fusarium</i> constitute a concern group of mycotoxins contaminating food products. In C?te d'Ivoire, data on the sanitary quality of marketed Moringa powder remains unknown. This study was carried out to characterize molds of the genus <i>Fusarium</i> isolated from <i>Moringa</i> powder by detecting the presence of a gene encoding the biosynthesis of fumonisins. A total of 192 <i>Moringa</i> leaf powder samples of approximately 250g each were taken from various sales sites in Abidjan markets including Abobo, Adjam¨¦, Koumassi and Yopougon. The isolation and purification of the <i>Fusarium </i>strains were carried out on Sabouraud¡¯s medium supplemented with 10 ¦Ìg / mL of chloramphenicol. The Polymerase Chain Reaction (PCR) method was used for molecular identification of <i>Fusarium</i> strains and the detection of the presence of the <i>Fum13</i> gene involved in the fumonisins biosynthesis. The contamination rate of <i>Moringa</i> powder by <i>Fusarium</i>-type molds was 15.43%. The predominantly isolated species was <i>Fusarium solani</i> (11.43%) followed by <i>Fusarium </i>sp (4%). The presence of the <i>Fum13</i> gene was detected in 62.96% (17/27) of the <i>Fusarium</i> strains isolated. The presence of molds of the genus <i>Fusarium </i>and the detection of a gene coding for the fumonisins biosynthesis, requires the implementation of health safety measures during the manufacturing process of <i>Moringa</i> powder.},
doi={10.12691/ajmr-9-3-5}
publisher={Science and Education Publishing}
}