eng Science and Education Publishing American Journal of Microbiological Research 2328-4137 2021-03-18 9 1 14 24 10.12691/ajmr-9-1-3 AJMR2021913 article Sahar Saad Allah Amin 1 Nada A. Abdel-Aziz 2 Wael S. El-Sayed 3 4 Manal Ibrahim Hanafi Mahmoud 5 6 Eman Mohamed Sharaf Elsayed 7 8 Rehab A. Eltahlawi emansharaf@yahoo.com, emsharaf@taibah.edu.sa 8 9 Microbiology and Immunology Department, Faculty of Medicine, Ain Shams University, Egypt Medical Microbiology and Immunology Department, Faculty of Medicine, Suhag University, Egypt Biology Department, Faculty of Science, Taibah University, Medina, KSA Department of Community Medicine, Ibn Sinai National College for Medical Studies, Jeddah, KSA Department of Microbiology and Immunology, College of Medicine, Taibah University, Kingdom of Saudi Arabia Background: Gram-positive uropathogens have become common, associated with serious underlying illnesses and increasingly resistant to available antibiotics. Objectives: The goal of this study was to investigate the incidence and risk factors of gram-positive cocci UTIs in hospitalized patients in Medina, KSA and their susceptibility patterns to widely used antimicrobial agents. Methods: During a 12-month study, 165 clinical isolates of gram-positive cocci were recovered from 1137 culture-positive urine specimens at a tertiary hospital. Antimicrobial susceptibility of gram-positive cocci isolates was tested with the disk diffusion and E test methods. Molecular typing of some VRE isolates was done to detect the predominant Van genotypes. Results: Out of 8600 reviewed cases, 1137 (13.2%) were culture positive, 165 cases (14.5%) were gram positive cocci. E.faecalis formed 53.3% (88/165) of isolated gram-positive cocci, followed by E faecium (17.6%), S.agalactiae (23.6%) and S. aureus (5.5%). Multidrug resistant positive cocci formed 9.7% of gram-positive isolates including VRE (8.5%) and MRSA (1.2%). 75% of E. faecalis and 50% of E. faecium isolates were sensitive to nitrofurantoin, all VRE strains were sensitive to linezolid. All S. aureus isolates were sensitive to cefazolin, nirtofurantoin, sulfamethoxazole and vancomycin. 75% of MRSA strains were sensitive to sulfamethoxazole and all were sensitive to vancomycin. All isolates of S. agalactiae were sensitive to cefazolin and nitrofurantoin, and all were resistant to Trimethoprim-sulfamethoxazole. Van B genotype was detected. Conclusion: Vancomycin and nitrofurantoin seem to be effective drugs for treatment of gram positive UTIs. vanB genotype was detected. http://pubs.sciepub.com/ajmr/9/1/3/ajmr-9-1-3.pdf urinary tract infection gram positive bacteria antibiotic resistance genotyping