@article{ajmr2020832,
author={{Dikwa, Karderam Bukar and Yahaya, Umar Abdullahi and Maikaje, Dominic Bawa and Suleiman, Ahmad Babangida},
title={Genetic Diversity of <i>Plasmodium</i> <i>falciparum</i> Isolated from Symptomatic and Asymptomatic Individuals in Parts of Kaduna Metropolis, Kaduna State, Nigeria},
journal={American Journal of Microbiological Research},
volume={8},
number={3},
pages={83--92},
year={2020},
url={http://pubs.sciepub.com/ajmr/8/3/2},
issn={2328-4137},
abstract={Malaria due to <i>Plasmodium falciparum</i> is still a major public health problem and cause of high morbidity and mortality in Nigeria despite many efforts and interventions programmes put in place to control malaria, with transmission occurring throughout the year. Characterization of <i>P. falciparum</i> isolates from different geographical locations of Nigeria could provide the much needed information on the genetic composition of <i>P. falciparum</i> natural populations with regard to three polymorphic genes: <i>Merozoite Surface Protein 1 (MSP 1), Merozoite Surface Protein (MSP 2) </i>and <i>Glutamate-Rich Protein</i> (<i>GLURP</i>) genes, the findings of this study will assist in adopting more strategies and intervention programmes aim at controlling <i>P. falciparum</i> infection in Nigeria. This study was conducted to determine the genetic diversity and elucidate possible genomic variation between isolates of <i>P. falciparum </i>from symptomatic and asymptomatic malaria cases in Kaduna metropolis. <i>Plasmodium falciparum</i> genomic DNA was extracted from 42 positive blood samples collected onto whatman¡¯s filter paper using the phenol-chloroform DNA extraction method. Genotyping of <i>P. falciparum </i>was based on the amplification of <i>MSP</i> 1, <i>MSP</i> 2 and <i>glurp</i> genes. Analysis of PCR products of <i>P. falciparum</i> isolates shows the presence of 33 alleles spread across <i>MSP</i> 1, <i>MSP</i> 2 and <i>glurp</i> genes at a frequency of 5, 24 and 4 respectively. Detection of the three genes; (<i>MSP</i>1); MAD20 and KI, (<i>MSP</i>2); FC27 and 3D7/ICI and (<i>glurp</i>) gene and the occurrence of single and multiple genotypic infection recorded showed the diverse genetic composition of <i>P. falciparum </i>population, an indication of the endemicity of malaria. This information is essential for effective malaria control programme.},
doi={10.12691/ajmr-8-3-2}
publisher={Science and Education Publishing}
}