@article{ajmr2020813,
author={{EL-Amir, Mostafa I. and R, Asmaa Gaber and A, Mohamed Yousef and Gamal, Tunis and El-Feky, Mohamed Ali},
title={Detection of Biofilm in Surgical Site Infection by Microtiter Plate Method and Its Correlation icaD, and icaA genes in <i>Staphylococcus</i> Spp},
journal={American Journal of Microbiological Research},
volume={8},
number={1},
pages={19--23},
year={2020},
url={http://pubs.sciepub.com/ajmr/8/1/3},
issn={2328-4137},
abstract={Purpose: To determine the bacteriological pattern of bacteria causing surgical site infection (SSI) in Qena University Hospitals and detection of biofilm producing organisms. Methodology: Wound swab from 114 patients with SSI were collected for identification of bacteria and detection of biofilm producing organisms by microtiter plate (MTP) method and tube method (TM). icaA and icaD genes were detected in <i>Staphylococcus</i><i> </i><i>spp.</i> by polymerase chain reaction (PCR) from extracted bacterial DNA Principal Findings:Patients mean age was 33.6 ¡À 12.7 years. 56.1% of the patients were enrolled to emergent surgeries. Diabetes mellitus was the most common risk factor detected in 17.5%. The highest infection rates were noticed after appendectomy, then followed by cholecystectomy (45.6% and 36.8% respectively). Most of bacteria isolated were gram-negative bacteria 67.2%. <i>Escherichia</i><i> </i><i>coli</i> was the most frequent bacteria (32.7%), followed by <i>Klebsiella</i><i> </i><i>pneumonia</i><i> </i>(26.2%), <i>Staphylococcus</i><i> </i><i>aureus</i> (22.9%), <i>Staphylococcus</i><i> </i><i>epidermidis</i> (9.8%), <i>Pseudomonas</i><i> </i><i>aeruginosa</i>(5.7%), <i>then</i><i> </i><i>Proteus</i><i> </i><i>spp</i><i> </i>(2.5%)<i>.</i> Significant biofilm production were detected between <i>Staphylococcus</i><i> </i><i>aureus</i> and<i> </i><i>E.</i><i> </i><i>coli</i>spp by MTP method (P value &lt;0.001 and 0.001 respectively). Significant difference between MTP method and TM in detection of biofilm producers (77.8% and 32.7% respectively), (P value &lt;0.001). icaD gene was detected in 62.5% of <i>Staphylococcus</i><i> </i>strains, While all <i>Staphylococcus</i><i> </i><i>strains</i><i> </i>were negative for icaA gene. All positive strains for icaD gene were strong biofilm producer by MTP method. Conclusion: MTP was better than TM in detection of biofilm formation. icaD gene were positive in strong biofilm producer <i>Staphylococcus</i> by MTP method while icaA not.},
doi={10.12691/ajmr-8-1-3}
publisher={Science and Education Publishing}
}