eng Science and Education Publishing American Journal of Microbiological Research 2328-4137 2019-12-08 7 4 118 121 10.12691/ajmr-7-4-3 AJMR2019743 article Athar Salah ELdin Moustafa sandi185315@gmail.com 1 Saada Mohamed Nour Kheiry 2 Department of Microbiology, University of Bahri, Khartoum, Sudan Department of Microbiology University of Bahri Objective: This study had been done to investigate the epidemiological role of the low hygiene and existence of domestic cats in the incidence of asymptomatic toxoplasmosis in human. Material and method: Serodiagnosis of Toxoplasma gondii using toxo (IgM, IgG) combo rapid test to detect the immunoglobulin M and G in sera collected from 60 restaurant male and female workers ranging between (15-69) years in Khartoum town. Result: 20% of participant gave positive results for immunoglobulin against Toxoplasma, three individual were IgM positive (5%). 9 individual were IgG positive (15%) only 2 female were diagnosed and they were negative. The highest positivity was given by the age group (37-47) years 50%. 5% were IgM positive and this means they are recently infected. 15% were IgG positive and this means they were either infected or have been immunized after infection. Conclusion: Low hygiene, existence of domestic cats and the Sudanese habit of eating raw meat have a potential epidemiological role in the transmission of toxoplasmosis to human. http://pubs.sciepub.com/ajmr/7/4/3/ajmr-7-4-3.pdf toxoplasmosis immunoglobulin Toxoplasma gondii