@article{ajmr2019742,
author={{Ahmed, Nehal and Rizk, Dina E and Said, Manal El and Barwa, Rasha MF and Elsokary, Mohammed Adel and Hassan, Ramadan HI},
title={Molecular Identification and Virulence Factors Determination in <i>Candida </i>Species Isolated from Egyptian Patients},
journal={American Journal of Microbiological Research},
volume={7},
number={4},
pages={108--117},
year={2019},
url={http://pubs.sciepub.com/ajmr/7/4/2},
issn={2328-4137},
abstract={Candida-related infections are becoming a universal threat to the health of human who undergo immunosuppressive therapy or aggressive medical intervention. <b>Objectives</b>: The aim was to study the distribution of <i>Candida</i> species among winter and summer seasons and to determine the expression of their virulence factors. <b>Methods:</b> A total of 164 <i>Candida</i> isolates were collected from clinical specimens at Mansoura University Hospitals. <i>Candida</i> species were identified by polymerase chain reaction (PCR). Extracellular phospholipase, secretory aspartyl proteinase (SAP) and coagulase enzymes and biofilm formation were determined. <i>SAP 9 </i>and<i> 10</i> genes were detected by PCR. <b>Results</b>: Non-albicans (NAC) isolates<i> </i>were more dominant than <i>C. albicans </i>isolates<i> </i>(P value &lt; 0.0001). <i>C. tropicalis </i>was the most prevalent (59.2%) followed by<i> C. albicans</i> (31.1%), then <i>C. glabrata</i>, <i>C. krusie, </i>unidentified NAC and <i>C. kefyr </i>in<i> </i>3.7%, 2.4%, 2.4% and 1.2% respectively. Extracellular phospholipase activity was detected in 31.7% of <i>Candida</i> isolates. All C<i>. albicans</i> had phospholipase activity (100%) and one isolate of <i>C. tropicalis </i>was positive while other species were negative. SAPs activities were determined in 61.6% of <i>Candida</i> isolates and were detected in 70.1% and 62.7% among  <i>C. tropicalis</i> and<i> C.</i><i> </i><i>albicans</i> isolates respectively.<i> SAP9 </i>and<i> SAP 10 </i>genes were detected in 27.7% and 12.9% of <i>Candida </i>isolates showed positive SAPs activity respectively and they were all <i>C. albicans</i> strains. Other species did not harbor either <i>SAP9 </i>or <i>SAP10.</i> Coagulase activity was detected in 80.4% of <i>Candida</i> isolates<i> </i>with higher activity in<i> C. albicans </i>(88.2%), followed by <i>C. tropicalis </i>(81.4%), then other NAC isolates. Biofilm formation was determined in 69.5% of <i>Candida</i> isolates and was more prevalent in C<i>. tropicalis </i>(82.5%) followed by <i>C. albicans </i>(19.6%), <i>C. krusie </i>(100%), unidentified <i>NAC</i> (75%), <i>C. glabrata </i>(33.3%) and<i> C. kefyr </i>(50%). <b>Conclusion:</b> NAC with a preponderance of <i>C. tropicalis</i> was the most common isolated <i>Candida</i> species. Biofilm production, proteinase, phospholipases and coagulase enzymes were observed in both <i>C. albicans</i> and NAC.<i> SAP9 </i>and <i>SAP</i> 10<i> </i>genes were detected only in <i>C. albicans</i> strains.},
doi={10.12691/ajmr-7-4-2}
publisher={Science and Education Publishing}
}