@article{ajmr2019721,
author={{O., Ajayi A. and A., Osanyinlusi S. and B., Ogeneh and A., Ojerinde O. and J., Oladeji S.},
title={Antibiotic Resistance Patterns among Gram-negative Bacteria from Patients with Urinary Tract Infection at a Healthcare Center in Ekiti-State, Nigeria},
journal={American Journal of Microbiological Research},
volume={7},
number={2},
pages={37--44},
year={2019},
url={http://pubs.sciepub.com/ajmr/7/2/1},
issn={2328-4137},
abstract={Urinary Tract Infections (UTIs) are huge health burden. This study determined the current incidence of antibiotic resistance among Gram-negative bacteria from patients with UTI at a health center in Ado Ekiti, Nigeria. One hundred and twenty two mid-stream urine samples were collected and cultured. Sixty-four samples (52.4%) grew significant bacteria. Gram-negative bacterial colonies were picked per plate, identified and tested for susceptibility to the following antibiotics: Ertapenem (10¦Ìg), meropenem (10¦Ìg), ceftazidime (30¦Ìg), ceftriaxone (30¦Ìg), gentamicin (10¦Ìg), ampicillin (10¦Ìg), tetracycline (30¦Ìg), norfloxacin (10¦Ìg) and pefloxacin (5¦Ìg). Thirty-nine isolates were selected for extended spectrum beta-lactamase production using the double disk synergy test. All the bacteria were tested for biofilms and haemolysis. Sixty-four Gram-negative bacterial isolates were recovered from 64 samples. The bacteria recovered included <i>K. pneumonia, K. oxytoca, P. vulgaris, E. coli, P. mirabilis </i>and<i> P. aeruginosa. </i>Overall, the bacteria showed the highest resistance to ampicillin 60/64 (94.5%) and least resistance to meropenem 18/64 (28.1%). All the bacteria tested for ESBL production were confirmed as ESBL producers. Twenty-two (34.3%) isolates were strong biofilm producers while 19 isolates were haemolytic (29.7%). These findings have serious implications to medical care and public health. Strict antibiotic stewardship should be enforced.},
doi={10.12691/ajmr-7-2-1}
publisher={Science and Education Publishing}
}