@article{ajmr2019715,
author={{Chemitei, Kipkogei and Amendi, Makumba B. and Mwamburi, Lizzy A. and Ochuodho, Julius Onyango},
title={Bio-Control of Net-Blotch and Scald Pathogens of Barley Using <i>Paenibacillus Polymyxa </i>KAI245 Isolated from Sorghum Rhizosphere in Western Kenya},
journal={American Journal of Microbiological Research},
volume={7},
number={1},
pages={28--36},
year={2019},
url={http://pubs.sciepub.com/ajmr/7/1/5},
issn={2328-4137},
abstract={Net-blotch and scald are important foliar diseases of barley. In the present study, the biocontrol activity of <i>Paenibacillus polymyxa </i>KaI245 was evaluated against <i>Drechsclera teres </i>f. sp <i>teres </i>and <i>Rhynchosporium commune </i>causing net-form-net-blotch and scald respectively. <i>In-vitro</i> efficacy of the bacterial isolate entailed dual culture technique, use of cell-free supernatant and test for volatile-compounds-mediated inhibition. Greenhouse studies were further conducted to evaluate the efficacy of crude bacterial extracts against net-blotch pathogen in barley plants. In dual culture technique, the mycelial growth of <i>D. teres </i>f. sp <i>teres </i>was impeded by approximately 47.3% while there was no any observable effect in <i>R. commune </i>colonies<i>.</i> Food-poison technique was u sed to test the antifungal activity of cell-free supernatant. The cell free supernatant inhibited the growth of <i>D. teres </i>by approximately 24.1%. <i>R. commune</i> colonies were impeded by 52.9% via volatile organic compounds while <i>D. teres </i>f. sp <i>teres </i>remained unaffected. Greenhouse studies showed decreased disease incidence (50%) in the crude-extract-treated barley leaves inoculated with <i>D. teres</i>. <i>In-vitro </i>studies revealed that greater inhibition is imparted by live bacterial cells. The bacterium has the potential to be used as a biocontrol agent against the tested pathogens of barley. Use of bio-control agents to manage crop diseases is one of the alternatives set to replace chemical fungicides that are saddled with lots of negativity due to their hazardous environmental impact.},
doi={10.12691/ajmr-7-1-5}
publisher={Science and Education Publishing}
}