@article{ajmr2018635,
author={{Almofti, Yassir A. and Abd-elrahman, Khoubieb Ali and Gassmallah, Sahar Abd Elgadir and Salih, Mohammed Ahmed},
title={Multi Epitopes Vaccine Prediction against Severe Acute Respiratory Syndrome (SARS) Coronavirus Using Immunoinformatics Approaches},
journal={American Journal of Microbiological Research},
volume={6},
number={3},
pages={94--114},
year={2018},
url={http://pubs.sciepub.com/ajmr/6/3/5},
issn={2328-4137},
abstract={Efforts for developing vaccine against Severe Acute Respiratory Syndrome coronavirus (SARS-CoV) is crucial in prevention of SARS re-emergence. The global outbreak of SARS was contained since 2003. However concerns remain over the possibility of future recurrences, especially with recent reports of laboratory-acquired infections and the presence of sporadic cases, raising a serious concern. SARS-CoV spike S protein (1255aa) is an important target in developing safe and effective vaccines. In this study multiple bio-informatics and immuno-informatics implementation tools from NCBI and IEDB were used for epitopes prediction from spike S protein. The predicted epitopes were further assessed for population coverage against the whole world population. Our results demonstrated that the epitopes <SUB>38-</SUB><b>RGVYYPDEI</b><SUB><b>-</b></SUB><SUB>46</SUB><b>, </b><SUB>200-</SUB><b>YQPIDVVRD</b><SUB>-208</SUB><b> </b>and<b> </b><SUB>388-</SUB><b>VVKGDDVRQ</b><SUB>-396</SUB> elicit and stimulate B cell since they got higher score in Emini and Kolaskar and tongaonker software. For T-cell: the epitopes <SUB>47-</SUB><b>FRSDTLYLT</b><SUB>-55, 195-</SUB><b>YVYKGYQPI</b><SUB><b>-</b></SUB><SUB>203</SUB> and <SUB>880-</SUB><b>FAMQMAYRF</b><SUB>-888</SUB> were found to interact with both MHC-1 and MHC-II alleles. Moreover <SUB>851-</SUB><b>MIAAYTAAL</b><SUB>-859</SUB> showed higher affinity to MHC-1 alleles while <SUB>782-</SUB><b>FNFSQILPD</b><SUB>-790</SUB><b> </b>interacted only with MHC-II alleles. The population coverage epitope set for MHC-1 and MHC-II predicted epitopes was <b>82.16% </b>and<b> 99.97% </b>respectively. All predicted epitopes against T cell (MHC-I/MHC-II) demonstrated strong potentiality as promising peptides vaccine<b> </b>with<b> </b>population coverage epitope set against the whole world of <b>100%</b>. Taken together eight epitopes were proposed to interact with B and T cells and act as peptide vaccine against SARS-CoV virus. In vitro and in vivo studies are recommended to prove the effectiveness of these epitopes as a peptide vaccine.},
doi={10.12691/ajmr-6-3-5}
publisher={Science and Education Publishing}
}