@article{ajmr2018612,
author={{S., Jamal Bayed and Bolad, Ahmed K.},
title={The Effect of Transfer Factor as Immunoprotective in Mice Challenged with <i>Mycobacteriu</i>m <i>tuberculosis</i>},
journal={American Journal of Microbiological Research},
volume={6},
number={1},
pages={9--13},
year={2018},
url={http://pubs.sciepub.com/ajmr/6/1/2},
issn={2328-4137},
abstract={<b>Background:</b><b> </b>Transfer Factor (TF) is an immune modulator which stimulates the cellular arm of the immune system (killer lymphocytes), activates immune cytokine synthesis and regulates immune function [1]. TF is very effective in those diseases in which CMI plays a relevant role in protection and control of the disease, such as intracellular bacterial diseases (tuberculosis) [2]. TF are low molecular weight products from immune cells that are able to transmit the ability to express delayed-type hypersensitivity (DTH) and cell mediated immunity (CMI) from sensitized donors to non immune recipients [3]. <b>Objectives</b><b>:</b><b> </b>The aim of this experimental study is to determine the protective efficacy of transfer factor (TF) as immunotprotective for mice. <b>Materials</b><b> </b><b>and</b><b> </b><b>methods</b><b>:</b><b> </b>A total number of 82 mice were examined for their immunopotency and protective efficacy of Transfer factor (TF) against challenge dose of <i>M.</i><i> </i><i>tuberculosis</i> (10<SUP>7</SUP> CFU). A number of 20 mice were immunized with the attenuated strain of <i>M</i>. <i>bovis</i>, Bacillus Calmette-Gu¨Śrin (BCG). After 21 days of BCG spleens of 10 tuberculous mice were removed aseptically for the preparation of TF. To evaluate the effect of TF 3 groups of inbred BALB/c male mice were injected with TF and challenged with virulent <i>M.</i><i> </i><i>tuberculosis</i>, All mice with TF were tested for tuberculin skin test (TST) so as to determine susceptibility and resistance against tuberculosis, susceptible groups of mice were challenged with virulent <i>M.</i><i> </i><i>tuberculosis</i>.Followed by study of humoral response by immunization of a group of mice with immune sera and challenged with <i>M.</i><i> </i><i>tuberculosis</i> H37Rv strain. Followed by an experiment of group A and B for the susceptibility and resistance of the strains of mice. <b>Results:</b><b> </b>After three weeks of observations the mice of experiment were tested for tuberculin skin test and the results were positive. Effectiveness determination of TF as protective efficacy was (83.3%). Humoral immunity response against <i>M.</i><i> </i><i>tuberculosis</i> showed negative reaction hence mortality rate was 100%, group B mice were resistant for BCG (Swiss white strain). <b>Conclusions:</b> The results indicated that administration of murine transfer factor (mTF) extracted and prepared from spleen of animal model (mice) as immunoprotective for challenged mice of <i>M.</i><i> </i><i>tuberculosis</i> (H37Rv) showed a better results enhanced immune response in respect to delayed type hypersensitivity, survival rate and mortality rate suggesting that efficacy of mTF as immunotherapy for tuberculosis.},
doi={10.12691/ajmr-6-1-2}
publisher={Science and Education Publishing}
}