eng Science and Education Publishing American Journal of Microbiological Research 2328-4137 2017-06-12 5 2 44 50 10.12691/ajmr-5-2-4 AJMR2017524 article Angel Justiz-Vaillant angel.vaillant@sta.uwi.edu 1 Norma McFarlane-Anderson 2 Monica Smikle 3 Department of Para-clinical Sciences, The University of the West Indies, Trinidad and Tobago Department of Basic Medical Sciences, The University of the West Indies, Kingston, Jamaica Department of Microbiology, The University of the West Indies, Kingston, Jamaica The new contributions of this paper include the identification of bacterial Ig receptors as valuable reagents for the detection of Ig molecules in species of wild, domestic and laboratory animals. It is important to detect antibodies as markers of infection and zoonotic diseases. The techniques used to investigate the binding of the bacterial Ig receptors with immunoglobulins present in different specimens were established techniques such as ELISA and immunoblot analyses. In addition, the affinity chromatography allowed for the purification of immunoglobulins and their fragments. The use of commercially available conjugates of enzyme labelled proteins L, A, G and SpLA is discussed. http://pubs.sciepub.com/ajmr/5/2/4/ajmr-5-2-4.pdf staphylococcal protein A streptococcal protein G peptostreptococcal protein L bacterial Ig-receptor immunoglobulin protein LG protein AG protein LA chimeric protein LAG (SpLAG)