Science and Education Publishing American Journal of Microbiological Research 2328-4137 5 2 2017 4 10 Operative Techniques, Treatment, and Precaution of Common Complications of Orthotopic Rat Liver Transplantation 28 31 EN Mao-Ming Tang Yun-Bing Wang Jian-Ping Gong Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China AJMR2017521 10.12691/ajmr-5-2-1 2016 11 9 2017 2 2 2017 4 8 Objective This study investigates operative techniques, treatment, and precaution for common complications of orthotopic liver transplantation (OLT) in rats. Methods OLT was performed in 110 rats through modified two-cuff method. Operative techniques were concluded, and causes, treatment, and precaution of common complications were analyzed. Results In operation phase, main causes for rat death included anesthesia, airway obstruction, pneumothorax, hemorrhagic shock, long anhepatic time, and air embolism. After surgery, main causes for rat death were as follows: stoma bleeding, infection, obstruction, and necrosis of biliary tract and poor hepatic function restoration. Conclusion Improving operative techniques, modifying operative methods, and familiarizing with common complications can reduce occurrence of complications, heighten operative successful rate, and establish more stable models of OLT in rats.