eng Science and Education Publishing American Journal of Microbiological Research 2328-4137 2016-12-23 4 6 172 177 10.12691/ajmr-4-6-3 AJMR2016463 article Mohamed Farouk mohamed.f.mirghani@gmail.com 1 Abraham Tesfaye 2 Mekuria Lakew 2 Department of Microbial Cellular & Molecular Biology, Addis Ababa University, Addis Ababa, Ethiopia Addis Ababa City Administration Health Research Bureaus, Addis Ababa, Ethiopia Hepatitis G virus or GB virus C (GBV-C) is a virus in the Flaviviridae family that does not cause any disease, but from various epidemiological observations was found to improve the clinical outcomes of HIV infection and treatment. It has the same mode of transmission with HIV. In Ethiopia, there has not been any study made on the prevalence or genotyping of GBV-C in circulation. Therefore no information is available. To fill this gap prevalence study was conducted on HIV patients under treatment. Eighty one serum samples were collected from patients on follow up study at the Addis Ababa Regional Laboratories and Research Institutes. RNA was extracted, reversed transcribed, and amplified by Real Time polymerase chain reaction (PCR), using primers for 5- untranslated region (5-UTR) of the GBV-C. Results showed 7 (8.6%) out of the 81 of HIV patients to be coinfected with GBV-C. Comparison of the mean CD4 count of the coinfected subject was found to be significantly different from the GBV-C negative patients at (P < 0.05). Further studies on clinical, immunological and genotypic analysis on larger samples are under investigation. http://pubs.sciepub.com/ajmr/4/6/3/ajmr-4-6-3.pdf GB Virus C HIV HAART PCR HGV