eng Science and Education Publishing American Journal of Microbiological Research 2328-4137 2015-07-08 3 4 129 134 10.12691/ajmr-3-4-2 AJMR2015342 article Sesilia Rante Pakadang 1 Chatarina Umbul Wahjuni 2 Hari Basuki Notobroto 2 Dwi Winarni 3 Ressy Dwiyanti 4 5 Yadi 6 6 Muhammad Sabir 6 6 Mochammad Hatta hattaram@indosat.net.id 6 Health Technology Department, Ministry of Health, Makasssar, Indonesia Faculty of Public Health, Airlangga University, Surabaya, Indonesia Faculty of Life Sciences and Technology, Airlangga University, Surabaya, Indonesia Department of Microbiology, Faculty of Medicine, Tadulako University, Palu, Indonesia Department of Microbiology and Immunology, Faculty of Medicine, Mulawarman University, Samarinda, Indonesia Aim, The aim of this study to investigate the immunonomodulator effects of miana leaves (Coleus scutellarioides (L) Benth) on prevention of tuberculosis in wistar rats, Method, Samples of white male Wistar rats were divided into 4 groups, the samples were treated with miana leaves extract (EDM) and then infected by intra tracheal M. tuberculosis H37Rv strain and subsequently given a placebo, EDM and GAB (combined Rifampicin and EDM) and healthy control animals were treated with EDM. In this study we measure the level of Immunomodulatory parameter; the number of T-lymphocytes and CD4 T-cells measured by flowcytometry method, the levels of IFN-γ and TNF-α were measured by ELISA and the number of M. tuberculosis colonies derived from the rat lung in LJ media. Result, Results showed the increasing of T-lymphocytes, CD4 T-Cells, the levels of IFN-γ and TNF-α and decreasing in the number of M. tuberculosis colonies after EDM treatment. Conclusion, Miana leaves extract (Coleus scutellarioides, (L) Benth.) increased the number of T-lymphocytes, CD4 T-cell counts, levels of IFN-γ and TNF-α and decreased in the number of M. tuberculosis colony in infected wistar lung. http://pubs.sciepub.com/ajmr/3/4/2/ajmr-3-4-2.pdf immunomodulator miana leaves (Coleus scutellarioides (L) Benth) mycobacterium tuberculosis CD4 IFN-γ TNF-α